Characterization of Epoxide Hydrolase Activity in Alternaria Alternata F. Sp. Lycopersici. Possible Involvement in Toxin Production

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 1997 Jan 1

PMID 9608724

Citations 2

Authors

F Pinot

E D Caldas

C Schmidt

D G Gilchrist

A D Jones

C K Winter

B D Hammock

Affiliations

Soon will be listed here.

Abstract

Using trans-diphenylpropane oxide (tDPPO) as a substrate, we measured epoxide hydrolase (EH) activity in subcellular fractions of Alternaria alternata f. sp. lycopersici (Aal), a fungus that produces host-specific toxins. The activity was mainly (> 99.5%) located in the soluble fraction (100,000 x g supernatant) with the optimum pH at 7.4. An increase of toxin production between days 3 and 9 found in a Aal liquid culture over a 15 days period was concomitant with a period of high EH activity. EH activity remained constant during the same period in an Alternaria alternata culture, a fungus which does not produce toxin. In vivo treatment of Aal culture with the peroxisome proliferator clofibrate stimulated EH activity by 83% and enhanced toxin production 6.3 fold. Both 4-fluorochalcone oxide (4-FCO) and (2S,3S)-(-)-3-(4-nitrophenyl)-glycidol (SS-NPG) inhibited EH activity in vitro with a I50 of 23 +/- 1 microM and 72 +/- 19 microM, respectively. The possible physiological substrate 9,10-epoxystearic acid was hydrolyzed more efficiently by Aal sEH than the model substrates trans- and cis-stilbene oxide (TSO and CSO) and trans- and cis-diphenylpropane oxide (tDPPO and cDPPO).

Citing Articles

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Sinha N, Patra S, Ghosh S Front Microbiol. 2022; 13:847832.

PMID: 35479629 PMC: 9037145. DOI: 10.3389/fmicb.2022.847832.

Multiple epoxide hydrolases in Alternaria alternata f. sp. lycopersici and their relationship to medium composition and host-specific toxin production.

Morisseau C, Ward B, Gilchrist D, Hammock B Appl Environ Microbiol. 1999; 65(6):2388-95.

PMID: 10347018 PMC: 91353. DOI: 10.1128/AEM.65.6.2388-2395.1999.

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