Technical Note: Improved DNA Extraction from Ancient Bones Using Silica-based Spin Columns

Overview

Journal Am J Phys Anthropol

Specialty Social Sciences

Date 1998 May 19

PMID 9584894

Citations 158

Authors

D Y Yang

B Eng

J S Waye

J C Dudar

S R Saunders

Affiliations

Soon will be listed here.

Abstract

We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick3, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.

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