Identification of a Homolog of CcpA Catabolite Repressor Protein in Streptococcus Mutans

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1998 May 9

PMID 9573093

Citations 32

Authors

C L Simpson

R R Russell

Affiliations

Soon will be listed here.

Abstract

A locus containing a gene with homology to ccpA of other bacteria has been cloned from Streptococcus mutans LT11, sequenced, and named regM. Upstream of the regM gene, on the opposite strand, is a gene encoding an X-Pro dipeptidase, pepQ. A 14-bp palindromic sequence with homology to the consensus catabolite-responsive element sequence lay in the promoter region between the two genes. To study the function of regM, the gene was inactivated by insertion of an antibiotic resistance marker. Diauxic growth of S. mutans on a number of sugars in the presence of glucose was not affected by disruption of regM. The loss of RegM increased glucose repression of alpha-galactosidase, mannitol-1-P dehydrogenase, and P-beta-galactosidase activities. These results suggest that while RegM can affect catabolite repression in S. mutans, it does not conform to the model proposed for CcpA in Bacillus subtilis.

Citing Articles

Regulation of fatty acid biosynthesis by the global regulator CcpA and the local regulator FabT in Streptococcus mutans.

Faustoferri R, Hubbard C, Santiago B, Buckley A, Seifert T, Quivey Jr R Mol Oral Microbiol. 2014; 30(2):128-46.

PMID: 25131436 PMC: 4415630. DOI: 10.1111/omi.12076.

Modification of gene expression and virulence traits in Streptococcus mutans in response to carbohydrate availability.

Moye Z, Zeng L, Burne R Appl Environ Microbiol. 2013; 80(3):972-85.

PMID: 24271168 PMC: 3911228. DOI: 10.1128/AEM.03579-13.

Gene regulation by CcpA and catabolite repression explored by RNA-Seq in Streptococcus mutans.

Zeng L, Choi S, Danko C, Siepel A, Stanhope M, Burne R PLoS One. 2013; 8(3):e60465.

PMID: 23555977 PMC: 3610829. DOI: 10.1371/journal.pone.0060465.

Catabolite control protein A of Streptococcus suis type 2 contributes to sugar metabolism and virulence.

Tang Y, Wu W, Zhang X, Lu Z, Chen J, Fang W J Microbiol. 2013; 50(6):994-1002.

PMID: 23274986 DOI: 10.1007/s12275-012-2035-3.

CcpA-dependent carbohydrate catabolite repression regulates galactose metabolism in Streptococcus oligofermentans.

Cai J, Tong H, Qi F, Dong X J Bacteriol. 2012; 194(15):3824-32.

PMID: 22609925 PMC: 3416513. DOI: 10.1128/JB.00156-12.

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