Structural Features Determining Differential Receptor Regulation of Neuronal Ca Channels

Overview

Journal J Neurosci

Specialty Neurology

Date 1998 Jun 6

PMID 9570799

Citations 17

Authors

A A Simen

R J Miller

Affiliations

Soon will be listed here.

Abstract

Dihydropyridine-insensitive Ca channels are subject to direct receptor G-protein-mediated inhibition to differing extents. alpha1B channels are much more strongly modulated than alpha1E channels. To understand the structural basis for this difference, we have constructed and expressed various alpha1B and alpha1E chimeric Ca channels and examined their regulation by kappa-opioid receptors. Replacement of the first membrane-spanning domain of alpha1E with the corresponding region of alpha1B resulted in a chimeric Ca channel that was modulated by kappa-opioid receptors to a significantly greater extent than alpha1E. Transfer of the N terminus and I/II loop from alpha1B in addition to domain I resulted in a chimeric channel that was modulated to the same extent as alpha1B. Other regions of the molecule do not appear to contribute significantly to the degree of inhibition obtained, although the C terminus may contribute to facilitation.

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References

Boussif O, Zanta M, Behr J . Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold. Gene Ther. 1996; 3(12):1074-80. View

Patil P, De Leon M, Reed R, Dubel S, Snutch T, Yue D . Elementary events underlying voltage-dependent G-protein inhibition of N-type calcium channels. Biophys J. 1996; 71(5):2509-21. PMC: 1233739. DOI: 10.1016/S0006-3495(96)79444-4. View

Tallent M, Dichter M, Bell G, Reisine T . The cloned kappa opioid receptor couples to an N-type calcium current in undifferentiated PC-12 cells. Neuroscience. 1994; 63(4):1033-40. DOI: 10.1016/0306-4522(94)90570-3. View

Bean B . Whole-cell recording of calcium channel currents. Methods Enzymol. 1992; 207:181-93. DOI: 10.1016/0076-6879(92)07013-e. View

Miller R . Receptor-mediated regulation of calcium channels and neurotransmitter release. FASEB J. 1990; 4(15):3291-9. View

Horton R, Cai Z, Ho S, Pease L . Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. Biotechniques. 1990; 8(5):528-35. View

Herlitze S, Hockerman G, Scheuer T, Catterall W . Molecular determinants of inactivation and G protein modulation in the intracellular loop connecting domains I and II of the calcium channel alpha1A subunit. Proc Natl Acad Sci U S A. 1997; 94(4):1512-6. PMC: 19822. DOI: 10.1073/pnas.94.4.1512. View

Portoghese P, Lipkowski A, TAKEMORI A . Binaltorphimine and nor-binaltorphimine, potent and selective kappa-opioid receptor antagonists. Life Sci. 1987; 40(13):1287-92. DOI: 10.1016/0024-3205(87)90585-6. View

Chen J, DeVivo M, Dingus J, Harry A, Li J, Sui J . A region of adenylyl cyclase 2 critical for regulation by G protein beta gamma subunits. Science. 1995; 268(5214):1166-9. DOI: 10.1126/science.7761832. View

10.

Simonin F, Gaveriaux-Ruff C, Befort K, Matthes H, Lannes B, Micheletti G . kappa-Opioid receptor in humans: cDNA and genomic cloning, chromosomal assignment, functional expression, pharmacology, and expression pattern in the central nervous system. Proc Natl Acad Sci U S A. 1995; 92(15):7006-10. PMC: 41460. DOI: 10.1073/pnas.92.15.7006. View

11.

Ikeda S . Voltage-dependent modulation of N-type calcium channels by G-protein beta gamma subunits. Nature. 1996; 380(6571):255-8. DOI: 10.1038/380255a0. View

12.

Ho S, Hunt H, Horton R, Pullen J, Pease L . Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 1989; 77(1):51-9. DOI: 10.1016/0378-1119(89)90358-2. View

13.

Merkouris M, Mullaney I, Georgoussi Z, Milligan G . Regulation of spontaneous activity of the delta-opioid receptor: studies of inverse agonism in intact cells. J Neurochem. 1998; 69(5):2115-22. DOI: 10.1046/j.1471-4159.1997.69052115.x. View

14.

Kaneko S, Fukuda K, Yada N, Akaike A, Mori Y, Satoh M . Ca2+ channel inhibition by kappa opioid receptors expressed in Xenopus oocytes. Neuroreport. 1994; 5(18):2506-8. DOI: 10.1097/00001756-199412000-00025. View

15.

Zhang J, Ellinor P, Aldrich R, Tsien R . Multiple structural elements in voltage-dependent Ca2+ channels support their inhibition by G proteins. Neuron. 1996; 17(5):991-1003. DOI: 10.1016/s0896-6273(00)80229-9. View

16.

Pragnell M, De Waard M, Mori Y, Tanabe T, Snutch T, Campbell K . Calcium channel beta-subunit binds to a conserved motif in the I-II cytoplasmic linker of the alpha 1-subunit. Nature. 1994; 368(6466):67-70. DOI: 10.1038/368067a0. View

17.

Marchetti C, Carbone E, Lux H . Effects of dopamine and noradrenaline on Ca channels of cultured sensory and sympathetic neurons of chick. Pflugers Arch. 1986; 406(2):104-11. DOI: 10.1007/BF00586670. View

18.

Yasuda K, Raynor K, Kong H, Breder C, Takeda J, Reisine T . Cloning and functional comparison of kappa and delta opioid receptors from mouse brain. Proc Natl Acad Sci U S A. 1993; 90(14):6736-40. PMC: 47007. DOI: 10.1073/pnas.90.14.6736. View

19.

Ikeda S . Double-pulse calcium channel current facilitation in adult rat sympathetic neurones. J Physiol. 1991; 439:181-214. PMC: 1180105. DOI: 10.1113/jphysiol.1991.sp018663. View

20.

Qin N, Platano D, Olcese R, Stefani E, Birnbaumer L . Direct interaction of gbetagamma with a C-terminal gbetagamma-binding domain of the Ca2+ channel alpha1 subunit is responsible for channel inhibition by G protein-coupled receptors. Proc Natl Acad Sci U S A. 1997; 94(16):8866-71. PMC: 23172. DOI: 10.1073/pnas.94.16.8866. View