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Purification and Molecular Cloning of a Chitinase Expressed in the Hepatopancreas of the Penaeid Prawn Penaeus Japonicus

Overview
Specialties Biochemistry
Biophysics
Date 1998 Apr 16
PMID 9540789
Citations 9
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Abstract

A protein with chitinase activity was purified from the hepatopancreas of the penaeid prawn Penaeus japonicus, and the amino acid sequences of several amino acid fragments were determined. A cDNA clone was isolated and sequenced which contained the coding sequence of the enzyme. The conceptually translated protein (named Pjchi-3) was a member of the chitinase family based on sequence similarities to chitinases of non-crustacean species, as was the case with Pjchi-1 and 2, two chitinase homologues previously isolated from P. japonicus.

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