Purification and Molecular Cloning of a Chitinase Expressed in the Hepatopancreas of the Penaeid Prawn Penaeus Japonicus
Overview
Biophysics
Authors
Affiliations
A protein with chitinase activity was purified from the hepatopancreas of the penaeid prawn Penaeus japonicus, and the amino acid sequences of several amino acid fragments were determined. A cDNA clone was isolated and sequenced which contained the coding sequence of the enzyme. The conceptually translated protein (named Pjchi-3) was a member of the chitinase family based on sequence similarities to chitinases of non-crustacean species, as was the case with Pjchi-1 and 2, two chitinase homologues previously isolated from P. japonicus.
Chen Z, Zhu S, Feng B, Zhang M, Gong J, Chen H Mar Biotechnol (NY). 2024; 26(3):511-525.
PMID: 38748059 DOI: 10.1007/s10126-024-10314-y.
Modulation of host lipid metabolism by virus infection leads to exoskeleton damage in shrimp.
Wang X, Ding M, Gao J, Zhao L, Cao R, Wang X PLoS Pathog. 2024; 20(5):e1012228.
PMID: 38739679 PMC: 11115362. DOI: 10.1371/journal.ppat.1012228.
Cloning, characterization, and expression analysis of the gene family in .
Chen L, Hua Y, Ji W, Wang J, Zhao H, Wang Z PeerJ. 2023; 11:e15045.
PMID: 36935907 PMC: 10022498. DOI: 10.7717/peerj.15045.
Physiological and muscle tissue responses in under hypoxic stress iTRAQ.
Chang F, Li N, Shi X, Olga V, Wang X, Diao X Front Physiol. 2022; 13:979472.
PMID: 36111157 PMC: 9468788. DOI: 10.3389/fphys.2022.979472.
Song Y, Villeneuve D, Toyota K, Iguchi T, Tollefsen K Environ Sci Technol. 2017; 51(8):4142-4157.
PMID: 28355071 PMC: 6135102. DOI: 10.1021/acs.est.7b00480.