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A Genetic Linkage Map of a Cichlid Fish, the Tilapia (Oreochromis Niloticus)

Overview
Journal Genetics
Publisher Oxford University Press
Specialty Genetics
Date 1998 Apr 16
PMID 9539437
Citations 66
Authors
T D Kocher
W J Lee
H Sobolewska
D Penman
B McAndrew
Affiliations
Soon will be listed here.
Abstract

We have constructed a genetic map for a tilapia, Oreochromis niloticus, using DNA markers. The segregation of 62 microsatellite and 112 anonymous fragment length polymorphisms (AFLPs) was studied in 41 haploid embryos derived from a single female. We have identified linkages among 162 (93.1%) of these markers. 95% of the microsatellites and 92% of the AFLPs were linked in the final map. The map spans 704 Kosambi cM in 30 linkage groups covering the 22 chromosomes of this species. Twenty-four of these linkage groups contain at least one microsatellite polymorphism. From the number of markers 15 or fewer cM apart, we estimate a total map length of approximately 1000-1200 cM. High levels of interference are observed, consistent with measurements in other fish species. This map is a starting point for the mapping of single loci and quantitative traits in cichlid fishes.

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References
1.
Wright J . Nucleotide sequence, genomic organization and evolution of a major repetitive DNA family in tilapia (Oreochromis mossambicus/hornorum). Nucleic Acids Res. 1989; 17(13):5071-9. PMC: 318095. DOI: 10.1093/nar/17.13.5071. View
2.
Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M . AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995; 23(21):4407-14. PMC: 307397. DOI: 10.1093/nar/23.21.4407. View
3.
Lincoln S, Lander E . Systematic detection of errors in genetic linkage data. Genomics. 1992; 14(3):604-10. DOI: 10.1016/s0888-7543(05)80158-2. View
4.
Hulbert S, Ilott T, Legg E, Lincoln S, Lander E, Michelmore R . Genetic analysis of the fungus, Bremia lactucae, using restriction fragment length polymorphisms. Genetics. 1988; 120(4):947-58. PMC: 1203586. DOI: 10.1093/genetics/120.4.947. View
5.
Kauffman E, Gestl E, Kim D, Walker C, Hite J, Yan G . Microsatellite-centromere mapping in the zebrafish (Danio rerio). Genomics. 1995; 30(2):337-41. DOI: 10.1006/geno.1995.9869. View