Enzymatic Action of Coliphage Omega8 and Its Possible Role in Infection

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1976 Sep 1

PMID 9521

Citations 5

Authors

P Prehm

K Jann

Affiliations

Soon will be listed here.

Abstract

The receptor of coliphage omega8 is the O-specific mannan of Escherichia coli O8 in which the trisaccharide alpha-mannosyl-1,2-alpha-mannosyl-1,2-mannose is joined through alpha-mannosyl-1,3-linkages. Coliphage omega8 produces an endo-alpha-1,3-mannosidase which destroys the receptor, liberating a series of oligosaccharides (repeating trisaccharide and multiples). The enzyme is an integral part of the phage particles and also occurs in a free form in the lysates. Phage particles hydrolyze alpha-1,3-mannosyl linkages in the lipopolysaccharide, the polysaccharide (mannan) moiety, and higher oligosaccharides with an efficiency decreasing in this order. No transmannosylation could be detected. Phage particles also degrade the receptor mannan on whole bacteria, as determined with 14C-labeled E. coli O8. The values of Km and Vmax were determined with omega8 particles and free enzymes using native lipopolysaccharide and its triethylammonium salt. The latter, which was obtained after electrodialysis, has a micellar weight of 2.5 X 10(5), whereas the native lipopolysaccharide forms supermicelles with micellar weights of several millions. With coliphage omega8 as enzyme and supermicellar lipopolysaccharide as substrate Km=5 X 10(-8) M was obtained. This, together with the fact that omega8 attaches irreversibly to E. coli O8, was used in proposing a hypothesis for the possible role of the enzyme in the first steps of infection with coliphage omega8.

Citing Articles

Bacteriophage-encoded virion-associated enzymes to overcome the carbohydrate barriers during the infection process.

Latka A, Maciejewska B, Majkowska-Skrobek G, Briers Y, Drulis-Kawa Z Appl Microbiol Biotechnol. 2017; 101(8):3103-3119.

PMID: 28337580 PMC: 5380687. DOI: 10.1007/s00253-017-8224-6.

Regulation of infection efficiency in a globally abundant marine Bacteriodetes virus.

Howard-Varona C, Roux S, Dore H, Solonenko N, Holmfeldt K, Markillie L ISME J. 2016; 11(1):284-295.

PMID: 27187794 PMC: 5335546. DOI: 10.1038/ismej.2016.81.

Bacteriophages and phage-derived proteins--application approaches.

Drulis-Kawa Z, Majkowska-Skrobek G, Maciejewska B Curr Med Chem. 2015; 22(14):1757-73.

PMID: 25666799 PMC: 4468916. DOI: 10.2174/0929867322666150209152851.

Polymannose O-antigens of Escherichia coli, the binding sites for the reversible adsorption of bacteriophage T5+ via the L-shaped tail fibers.

Heller K, Braun V J Virol. 1982; 41(1):222-7.

PMID: 7045389 PMC: 256742. DOI: 10.1128/JVI.41.1.222-227.1982.

Salmonella bacteriophage glycanases: endorhamnosidases of Salmonella typhimurium bacteriophages.

Svenson S, Lonngren J, Carlin N, Lindberg A J Virol. 1979; 32(2):583-92.

PMID: 387979 PMC: 353590. DOI: 10.1128/JVI.32.2.583-592.1979.

References

Fehmel F, Feige U, Niemann H, STIRM S . Escherichia coli capsule bacteriophages. VII. Bacteriophage 29-host capsular polysaccharide interactions. J Virol. 1975; 16(3):591-601. PMC: 354707. DOI: 10.1128/JVI.16.3.591-601.1975. View

Galanos C, LUDERITZ O . Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms. Eur J Biochem. 1975; 54(2):603-10. DOI: 10.1111/j.1432-1033.1975.tb04172.x. View

Prehm P, STIRM S, JANN B, Jann K . Cell-wall lipopolysaccharide from Escherichia coli B. Eur J Biochem. 1975; 56(1):41-55. DOI: 10.1111/j.1432-1033.1975.tb02205.x. View

Iwashita S, Kanegasaki S . Release of O antigen polysaccharide from Salmonella newington by phage epsilon 34. Virology. 1975; 68(1):27-34. DOI: 10.1016/0042-6822(75)90144-0. View

Garen A . Thermodynamic and kinetic studies on the attachment of T1 bacteriophage to bacteria. Biochim Biophys Acta. 1954; 14(2):163-72. DOI: 10.1016/0006-3002(54)90155-9. View

LOEB T, Zinder N . A bacteriophage containing RNA. Proc Natl Acad Sci U S A. 1961; 47:282-9. PMC: 221572. DOI: 10.1073/pnas.47.3.282. View

Garen A, PUCK T . The first two steps of the invasion of host cells by bacterial viruses. II. J Exp Med. 1951; 94(3):177-89. PMC: 2136106. DOI: 10.1084/jem.94.3.177. View

Stent G, WOLLMAN E . On the two step nature of bacteriophage absorption. Biochim Biophys Acta. 1952; 8(3):260-9. DOI: 10.1016/0006-3002(52)90041-3. View

Park J, Johnson M . A submicrodetermination of glucose. J Biol Chem. 1949; 181(1):149-51. View

10.

Thurow H, Niemann H, STIRM S . Bacteriophage-borne enzymes in carbohydrate chemistry. Part I. On the glycanase activity associated with particles of Klebsiella bacteriophage No. 11. Carbohydr Res. 1975; 41:257-71. DOI: 10.1016/s0008-6215(00)87024-x. View

11.

Bayer M, Starkey T . The adsorption of bacteriophage phi X174 and its interaction with Escherichia coli; a kinetic and morphological study. Virology. 1972; 49(1):236-56. DOI: 10.1016/s0042-6822(72)80026-6. View

12.

Reske K, Jann K . The O8 antigen of Escherichia coli. Structure of the polysaccharide chain. Eur J Biochem. 1972; 31(2):320-8. DOI: 10.1111/j.1432-1033.1972.tb02536.x. View

13.

Taylor K, Kwiatkowski B . Electron microscopic studies of Vi-phage II adsorption on Salmonella typhi. Acta Microbiol Pol. 1966; 15(1):27-34. View

14.

Kwiatkowski B, Taylor A . Two-step attachment of Vi-phage I to the bacterial surface. Acta Microbiol Pol A. 1970; 2(1):13-20. View

15.

Takeda K, Uetake H . In vitro interaction between phage and receptor lipopolysaccharide: a novel glycosidase associated with Salmonella phage epsilon15. Virology. 1973; 52(1):148-59. View

16.

Ribi E, Anacker R, Brown R, HASKINS W, Malmgren B, MILNER K . Reaction of endotoxin and surfactants. I. Physical and biological properties of endotoxin treated with sodium deoxycholate. J Bacteriol. 1966; 92(5):1493-509. PMC: 276450. DOI: 10.1128/jb.92.5.1493-1509.1966. View

17.

JANN B, Jann K, Schmidt G, ORSKOV I, ORSKOV F . Immunochemical studies of polysaccharide surface antigens of Escherichia coli 0100:K?(B):H2. Eur J Biochem. 1970; 15(1):29-39. DOI: 10.1111/j.1432-1033.1970.tb00972.x. View

18.

Fairbanks G, Steck T, Wallach D . Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971; 10(13):2606-17. DOI: 10.1021/bi00789a030. View

19.

Muhlradt P, Menzel J, Golecki J, Speth V . Outer membrane of salmonella. Sites of export of newly synthesised lipopolysaccharide on the bacterial surface. Eur J Biochem. 1973; 35(3):471-81. DOI: 10.1111/j.1432-1033.1973.tb02861.x. View

20.

Reske K, Wallenfels B, Jann K . Enzymatic degradation of O-antigenic lipopolysaccharides by coliphage omega 8. Eur J Biochem. 1973; 36(1):167-71. DOI: 10.1111/j.1432-1033.1973.tb02897.x. View