Directed Changes in the Number of Double-stranded RNA Genomic Segments in Bacteriophage Phi6

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1998 May 9

PMID 9520468

Citations 10

Authors

S Onodera

X Qiao

J Qiao

L Mindich

Affiliations

Soon will be listed here.

Abstract

Bacteriophage Phi6 has a genome of three segments of double-stranded RNA. The segments are designated S, M, and L. Each segment has a unique packaging site, pac, near the 5' end of the plus strand. The plus strands of the segments are normally packaged in the order S, M, L. Chimeras of segment M and S in which segment M is at the 5' end of the plus strand can be stably incorporated into the virion; however, an independent segment S must be included along with normal segment L, even if it contains no active genes. A chimera of segment M and S in which segment S is at the 5' end of the plus strand can be stably incorporated into the virion along with normal segment L to form a two-segment genome. A chimera of segments S, M, and L in which the packaging sequence is that of S can also form a stable nonsegmented genome. These findings are consistent with a model that we have proposed for the packaging of the Phi6 genome.

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