Direct Detection of Viable Bacteria, Molds, and Yeasts by Reverse Transcriptase PCR in Contaminated Milk Samples After Heat Treatment

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1998 Mar 21

PMID 9501455

Citations 7

Authors

M Vaitilingom

F Gendre

P Brignon

Affiliations

Soon will be listed here.

Abstract

A fast, sensitive, and target contaminant-modulable method was developed to detect viable bacteria, molds, and yeasts after heat treatment. By reverse transcriptase PCR with elongation factor gene (EF-Tu or EF-1 alpha)-specific primers, the detection level was 10 cells ml of milk-1. The simplicity and rapidity (4 h) of the procedure suggests that this method may be easily transposable to other foods and other contaminants.

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