Cloning and Characterization of a Novel Endothelin Receptor Subtype in the Avian Class

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1998 Apr 18

PMID 9501209

Citations 22

Authors

L Lecoin

T Sakurai

M T Ngo

Y Abe

M Yanagisawa

N M Le Douarin

Affiliations

Soon will be listed here.

Abstract

Endothelin 3 (EDN 3) and the endothelin receptor B (EDNRB) are involved in the development of neural crest and particularly of the melanocytes and the enteric nervous system. We reported previously that the avian EDNRB gene is expressed in the neural fold before crest cell migration and later on in all the neural crest derivatives except, at any developmental stage, in the melanocytic lineage. However, quail melanoblasts proliferate in response to EDN 3 stimulation in vitro. These observations prompted us to search for another type of endothelin receptor (EDNR). We report here the cloning by reverse transcriptase-PCR of an avian cDNA encoding a subtype of EDNR, which we have called EDNRB2, because its deduced amino acid sequence is more closely related to that of EDNRB than to either the mammalian EDNRA or to the Xenopus EDNRC. Its expression pattern differs from that of the "classical" avian EDNRB because it is strongly expressed in melanoblasts and melanocytes. EDNRB2 transcripts are also abundant in the liver and kidney. Our pharmacological studies showed that EDNRB2 binds with similar affinity to EDN 1, EDN 2, and EDN 3, further confirming that this receptor belongs to the B type, although it displays a low affinity for sarafotoxin-c, a known EDNRB-selective agonist.

Citing Articles

Genomic evidence for hybridization and introgression between blue peafowl and endangered green peafowl and molecular foundation of leucistic plumage of blue peafowl.

Wang G, Zhang X, Zhao X, Ren X, Chen A, Dai W Gigascience. 2025; 14.

PMID: 39965774 PMC: 11835448. DOI: 10.1093/gigascience/giae124.

Endothelin-3 Suppresses Luteinizing Hormone Receptor Expression by Regulating the cAMP-PKA Pathway in Hen Granulosa Cells.

Tai Y, Han D, Yang X, Cai G, Li H, Li J Curr Issues Mol Biol. 2024; 46(8):7832-7845.

PMID: 39194681 PMC: 11352380. DOI: 10.3390/cimb46080464.

Endothelin signaling in development.

Kanai S, Clouthier D Development. 2023; 150(24).

PMID: 38078652 PMC: 10753589. DOI: 10.1242/dev.201786.

Stripes and loss of color in ball pythons (Python regius) are associated with variants affecting endothelin signaling.

Dao U, Lederer I, Tabor R, Shahid B, Graves C, Seidel H G3 (Bethesda). 2023; 13(7).

PMID: 37191439 PMC: 10320763. DOI: 10.1093/g3journal/jkad063.

From Bipotent Neuromesodermal Progenitors to Neural-Mesodermal Interactions during Embryonic Development.

Kahane N, Kalcheim C Int J Mol Sci. 2021; 22(17).

PMID: 34502050 PMC: 8431582. DOI: 10.3390/ijms22179141.

References

Baroffio A, Dupin E, Le Douarin N . Clone-forming ability and differentiation potential of migratory neural crest cells. Proc Natl Acad Sci U S A. 1988; 85(14):5325-9. PMC: 281743. DOI: 10.1073/pnas.85.14.5325. View

Lahav R, Ziller C, Dupin E, Le Douarin N . Endothelin 3 promotes neural crest cell proliferation and mediates a vast increase in melanocyte number in culture. Proc Natl Acad Sci U S A. 1996; 93(9):3892-7. PMC: 39455. DOI: 10.1073/pnas.93.9.3892. View

Ziller C, Le Douarin N . Developmental potentials of enteric neural crest-derived cells in clonal and mass cultures. Dev Biol. 1994; 163(1):141-51. DOI: 10.1006/dbio.1994.1130. View

Lin H, Kaji E, Winkel G, IVES H, Lodish H . Cloning and functional expression of a vascular smooth muscle endothelin 1 receptor. Proc Natl Acad Sci U S A. 1991; 88(8):3185-9. PMC: 51410. DOI: 10.1073/pnas.88.8.3185. View

Schwinn D, Lomasney J, Lorenz W, Szklut P, Fremeau Jr R, Yang-Feng T . Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype. J Biol Chem. 1990; 265(14):8183-9. View

Lecoin L, Lahav R, Martin F, Teillet M, Le Douarin N . Steel and c-kit in the development of avian melanocytes: a study of normally pigmented birds and of the hyperpigmented mutant silky fowl. Dev Dyn. 1995; 203(1):106-18. DOI: 10.1002/aja.1002030111. View

Warner T, Allcock G, Corder R, Vane J . Use of the endothelin antagonists BQ-123 and PD 142893 to reveal three endothelin receptors mediating smooth muscle contraction and the release of EDRF. Br J Pharmacol. 1993; 110(2):777-82. PMC: 2175921. DOI: 10.1111/j.1476-5381.1993.tb13879.x. View

Fraser S, Bronner-Fraser M . Migrating neural crest cells in the trunk of the avian embryo are multipotent. Development. 1991; 112(4):913-20. DOI: 10.1242/dev.112.4.913. View

STONE J, Spirling L, Richardson M . The neural crest population responding to endothelin-3 in vitro includes multipotent cells. J Cell Sci. 1997; 110 ( Pt 14):1673-82. DOI: 10.1242/jcs.110.14.1673. View

10.

Ziller C, LE DOUARIN N . Developmental potentialities of cells derived from the truncal neural crest in clonal cultures. Brain Res Dev Brain Res. 1992; 66(1):1-10. DOI: 10.1016/0165-3806(92)90134-i. View

11.

Nambi P, Pullen M, Kumar C . Identification of a novel endothelin receptor in Xenopus laevis liver. Neuropeptides. 1994; 26(3):181-5. DOI: 10.1016/0143-4179(94)90128-7. View

12.

Brooks D, DePalma P, Pullen M, Gellai M, Nambi P . Identification and function of putative ETB receptor subtypes in the dog kidney. J Cardiovasc Pharmacol. 1995; 26 Suppl 3:S322-5. View

13.

Krystek Jr S, Patel P, Rose P, Fisher S, Kienzle B, Lach D . Mutation of peptide binding site in transmembrane region of a G protein-coupled receptor accounts for endothelin receptor subtype selectivity. J Biol Chem. 1994; 269(17):12383-6. View

14.

Karne S, Jayawickreme C, Lerner M . Cloning and characterization of an endothelin-3 specific receptor (ETC receptor) from Xenopus laevis dermal melanophores. J Biol Chem. 1993; 268(25):19126-33. View

15.

Takigawa M, Sakurai T, Kasuya Y, Abe Y, Masaki T, Goto K . Molecular identification of guanine-nucleotide-binding regulatory proteins which couple to endothelin receptors. Eur J Biochem. 1995; 228(1):102-8. DOI: 10.1111/j.1432-1033.1995.tb20236.x. View

16.

Kurihara Y, Kurihara H, Suzuki H, Kodama T, Maemura K, Nagai R . Elevated blood pressure and craniofacial abnormalities in mice deficient in endothelin-1. Nature. 1994; 368(6473):703-10. DOI: 10.1038/368703a0. View

17.

Panek R, Major T, Hingorani G, Doherty A, Taylor D, Rapundalo S . Endothelin and structurally related analogs distinguish between endothelin receptor subtypes. Biochem Biophys Res Commun. 1992; 183(2):566-71. DOI: 10.1016/0006-291x(92)90519-q. View

18.

Nataf V, Lecoin L, Eichmann A, Le Douarin N . Endothelin-B receptor is expressed by neural crest cells in the avian embryo. Proc Natl Acad Sci U S A. 1996; 93(18):9645-50. PMC: 38482. DOI: 10.1073/pnas.93.18.9645. View

19.

Erickson C, Duong T, Tosney K . Descriptive and experimental analysis of the dispersion of neural crest cells along the dorsolateral path and their entry into ectoderm in the chick embryo. Dev Biol. 1992; 151(1):251-72. DOI: 10.1016/0012-1606(92)90231-5. View

20.

Eichmann A, Marcelle C, Breant C, Le Douarin N . Two molecules related to the VEGF receptor are expressed in early endothelial cells during avian embryonic development. Mech Dev. 1993; 42(1-2):33-48. DOI: 10.1016/0925-4773(93)90096-g. View