Expression of Alphav Integrins and Vitronectin Receptor Identity in Breast Cancer Cells

Overview

Journal Br J Cancer

Specialty Oncology

Date 1998 Mar 4

PMID 9484807

Citations 33

Authors

T Meyer

J F Marshall

I R Hart

Affiliations

Soon will be listed here.

Abstract

In the present study we have used fluorocytometry and immunoprecipitation to characterize the expression of alphav-containing integrins in a panel of eight human breast cancer cell lines and one normal human mammary epithelial line. We show that the classical vitronectin receptor alphavbeta3 is expressed in only one cell line (MDA-MB-231), whereas alphavbeta5 is expressed on all breast cancer cell lines and alphavbeta1 is expressed on the majority. Using adherence assays to purified ligands in the presence and absence of function-blocking monoclonal antibodies, we have demonstrated that alphavbeta5 mediates adhesion to vitronectin in the majority of these cells. In one cell line, ZR75-1, alphavbeta1 contributes significantly to adhesion to immobilized vitronectin. The formation of focal adhesions containing the alphav and beta1 subunits on vitronectin is also demonstrated by indirect immunofluorescence.

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References

Nip J, Rabbani S, SHIBATA H, Brodt P . Coordinated expression of the vitronectin receptor and the urokinase-type plasminogen activator receptor in metastatic melanoma cells. J Clin Invest. 1995; 95(5):2096-103. PMC: 295806. DOI: 10.1172/JCI117897. View

Hynes R . Integrins: a family of cell surface receptors. Cell. 1987; 48(4):549-54. DOI: 10.1016/0092-8674(87)90233-9. View

Marshall J, Rutherford D, McCartney A, Mitjans F, Goodman S, Hart I . Alpha v beta 1 is a receptor for vitronectin and fibrinogen, and acts with alpha 5 beta 1 to mediate spreading on fibronectin. J Cell Sci. 1995; 108 ( Pt 3):1227-38. DOI: 10.1242/jcs.108.3.1227. View

Schnapp L, Hatch N, Ramos D, Klimanskaya I, Sheppard D, Pytela R . The human integrin alpha 8 beta 1 functions as a receptor for tenascin, fibronectin, and vitronectin. J Biol Chem. 1995; 270(39):23196-202. DOI: 10.1074/jbc.270.39.23196. View

Doerr M, Jones J . The roles of integrins and extracellular matrix proteins in the insulin-like growth factor I-stimulated chemotaxis of human breast cancer cells. J Biol Chem. 1996; 271(5):2443-7. DOI: 10.1074/jbc.271.5.2443. View

Agrez M, Bates R, Mitchell D, Wilson N, Ferguson N, Anseline P . Multiplicity of fibronectin-binding alpha V integrin receptors in colorectal cancer. Br J Cancer. 1996; 73(7):887-92. PMC: 2074269. DOI: 10.1038/bjc.1996.158. View

Marshall J, Hart I . The role of alpha v-integrins in tumour progression and metastasis. Semin Cancer Biol. 1996; 7(3):129-38. DOI: 10.1006/scbi.1996.0018. View

Stefansson S, Lawrence D . The serpin PAI-1 inhibits cell migration by blocking integrin alpha V beta 3 binding to vitronectin. Nature. 1996; 383(6599):441-3. DOI: 10.1038/383441a0. View

Yebra M, Parry G, Stromblad S, Mackman N, Rosenberg S, Mueller B . Requirement of receptor-bound urokinase-type plasminogen activator for integrin alphavbeta5-directed cell migration. J Biol Chem. 1996; 271(46):29393-9. DOI: 10.1074/jbc.271.46.29393. View

10.

Cheresh D, Smith J, Cooper H, Quaranta V . A novel vitronectin receptor integrin (alpha v beta x) is responsible for distinct adhesive properties of carcinoma cells. Cell. 1989; 57(1):59-69. DOI: 10.1016/0092-8674(89)90172-4. View

11.

Vogel B, Tarone G, Giancotti F, Gailit J, Ruoslahti E . A novel fibronectin receptor with an unexpected subunit composition (alpha v beta 1). J Biol Chem. 1990; 265(11):5934-7. View

12.

Bodary S, McLean J . The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line. J Biol Chem. 1990; 265(11):5938-41. View

13.

SOULE H, Maloney T, WOLMAN S, PETERSON Jr W, Brenz R, McGrath C . Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 1990; 50(18):6075-86. View

14.

Duffy M, Reilly D, OSullivan C, OHiggins N, Fennelly J, Andreasen P . Urokinase-plasminogen activator, a new and independent prognostic marker in breast cancer. Cancer Res. 1990; 50(21):6827-9. View

15.

Koukoulis G, Virtanen I, Korhonen M, Laitinen L, Quaranta V, Gould V . Immunohistochemical localization of integrins in the normal, hyperplastic, and neoplastic breast. Correlations with their functions as receptors and cell adhesion molecules. Am J Pathol. 1991; 139(4):787-99. PMC: 1886301. View

16.

Marshall J, Nesbitt S, Helfrich M, Horton M, Polakova K, Hart I . Integrin expression in human melanoma cell lines: heterogeneity of vitronectin receptor composition and function. Int J Cancer. 1991; 49(6):924-31. DOI: 10.1002/ijc.2910490621. View

17.

Gehlsen K, Davis G, Sriramarao P . Integrin expression in human melanoma cells with differing invasive and metastatic properties. Clin Exp Metastasis. 1992; 10(2):111-20. DOI: 10.1007/BF00114587. View

18.

Hynes R . Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992; 69(1):11-25. DOI: 10.1016/0092-8674(92)90115-s. View

19.

Pignatelli M, Cardillo M, Hanby A, Stamp G . Integrins and their accessory adhesion molecules in mammary carcinomas: loss of polarization in poorly differentiated tumors. Hum Pathol. 1992; 23(10):1159-66. DOI: 10.1016/0046-8177(92)90034-z. View

20.

Albelda S . Role of integrins and other cell adhesion molecules in tumor progression and metastasis. Lab Invest. 1993; 68(1):4-17. View