The H(+)-ATPase in the Plasma Membrane of Saccharomyces Cerevisiae is Activated During Growth Latency in Octanoic Acid-supplemented Medium Accompanying the Decrease in Intracellular PH and Cell Viability

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1998 Feb 17

PMID 9464423

Citations 24

Authors

C A Viegas

P F Almeida

M Cavaco

I Sa-Correia

Affiliations

Soon will be listed here.

Abstract

Saccharomyces cerevisiae plasma membrane H(+)-ATPase activity was stimulated during octanoic acid-induced latency, reaching maximal values at the early stages of exponential growth. The time-dependent pattern of ATPase activation correlated with the decrease of cytosolic pH (pHi). The cell population used as inoculum exhibited a significant heterogeneity of pHi, and the fall of pHi correlated with the loss of cell viability as determined by plate counts. When exponential growth started, only a fraction of the initial population was still viable, consistent with the role of the physiology and number of viable cells in the inoculum in the duration of latency under acid stress.

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