Cloning and Nucleotide Sequencing of a Staphylococcus Aureus Gene Encoding a Branched-chain-amino-acid Transporter

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1998 Feb 17

PMID 9464420

Citations 10

Authors

U Vijaranakul

A Xiong

K Lockwood

R K Jayaswal

Affiliations

Soon will be listed here.

Abstract

We recently characterized a transposon-induced NaCl-sensitive mutant of Staphylococcus aureus (U. Vijaranakul, M. J. Nadakavukaren, D. O. Bayles, B. J. Wilkinson, and R. K. Jayaswal, Appl. Environ. Microbiol. 63:1889-1897, 1997). To further characterize this mutant, we determined the nucleotide sequence at the insertion site of the transposon on the S. aureus chromosome. Nucleotide sequencing revealed a 1,326-bp open reading frame (ORF442) encoding a hydrophobic 442-amino-acid polypeptide with a calculated molecular mass of 49,058 Da. The hydrophilicity profile of the gene product revealed the existence of 12 hydrophobic domains predicted to form membrane-associated alpha-helices. Comparison of the amino acid sequence of ORF442 with amino acid sequences in the GenBank database showed extensive homology with the branched-chain-amino-acid transport genes of gram-positive and gram-negative bacteria. This is the first brnQ gene in staphylococci to be described.

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