Induction of TSC-22 by Treatment with a New Anti-cancer Drug, Vesnarinone, in a Human Salivary Gland Cancer Cell

Overview

Journal Br J Cancer

Specialty Oncology

Date 1998 Feb 12

PMID 9459148

Citations 14

Authors

H Kawamata

K Nakashiro

D Uchida

S Hino

F Omotehara

H Yoshida

M Sato

Affiliations

Soon will be listed here.

Abstract

We undertook the present study to clarify the molecular mechanism of the effect of a new anti-cancer drug, vesnarinone, on a human salivary gland cancer cell line, TYS. We isolated TSC-22cDNA as avesnarinone-inducible gene from a cDNA library constructed from vesnarinone-treated TYS cells. TSC-22 was originally reported as a transforming growth factor (TGF)-beta-inducible gene. The expression of TSC-22 was up-regulated within a few hours after treatment with vesnarinone and was continued for 3 days. The level of TSC-22 mRNA in TYS cells was continuously increased until the cells reached confluency. Furthermore, the induction of TSC-22 by vesnarinone was inhibited by treatment with cycloheximide. When we treated the cells with an antisense oligonucleotide against TSC-22 mRNA under quiescent conditions, the antisense oligonucleotide stimulated the growth of TYS cells; however, under growing conditions the antisense oligonucleotide did not affect cell growth. Furthermore, the antisense oligonucleotide suppressed the antiproliferative effect of vesnarinone. These results suggest that TSC-22 may be a negative growth regulator and may play an important role in the antiproliferative effect of vesnarinone.

Citing Articles

Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry.

Kamimura R, Uchida D, Kanno S, Shiraishi R, Hyodo T, Sawatani Y Int J Mol Sci. 2021; 22(20).

PMID: 34681573 PMC: 8536140. DOI: 10.3390/ijms222010913.

TSC-22 inhibits CSF-1R function and induces apoptosis in cervical cancer.

Cho M, Lee J, Shin M, Kim H, Choi Y, Rho S Oncotarget. 2017; 8(58):97990-98003.

PMID: 29228668 PMC: 5716708. DOI: 10.18632/oncotarget.20296.

Yeast two-hybrid screening identified WDR77 as a novel interacting partner of TSC22D2.

Li Q, Chen P, Zeng Z, Liang F, Song Y, Xiong F Tumour Biol. 2016; 37(9):12503-12512.

PMID: 27337956 DOI: 10.1007/s13277-016-5113-z.

Overexpression of TSC-22 (transforming growth factor- β-stimulated clone-22) causes marked obesity, splenic abnormality and B cell lymphoma in transgenic mice.

Uchida D, Kawamata H, Omotehara F, Miwa Y, Horiuchi H, Furihata T Oncotarget. 2016; 7(12):14310-23.

PMID: 26872059 PMC: 4924717. DOI: 10.18632/oncotarget.7308.

TSC-22 up-regulates collagen 3a1 gene expression in the rat heart.

Kelloniemi A, Aro J, Napankangas J, Koivisto E, Mustonen E, Ruskoaho H BMC Cardiovasc Disord. 2015; 15:122.

PMID: 26464165 PMC: 4604760. DOI: 10.1186/s12872-015-0121-2.

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