Enrichment of Carnitine Palmitoyltransferases I and II in the Contact Sites of Rat Liver Mitochondria

Overview

Journal Biochem J

Specialty Biochemistry

Date 1998 Feb 28

PMID 9425103

Citations 12

Authors

F Fraser

V A Zammit

Affiliations

Soon will be listed here.

Abstract

The submitochondrial distribution of the overt and latent carnitine palmitoyltransferases (CPT I and II respectively) of rat liver mitochondria were studied. Separation of outer and inner membranes, as well as of a fraction of intermediate density consisting of contact sites between the two membranes, was achieved, as judged by the distribution of marker enzymes. Both CPT I and CPT II were found to be enriched within the contact- site fraction of mitochondria. These data show that the two carnitine acyltransferases are distributed non-uniformly within their respective membranes, and that subpopulations of the two enzymes occur in close proximity within the mitochondrial membrane structure, while retaining their different accessibilities to cytosolic and matrix pools of metabolites. As the number of contact sites is known to vary with changes in the energy status of mitochondria, the possibility that such changes may acutely affect the proportion of CPT I within the distinctive lipid environment of the contact sites, and thus its overall kinetic characteristics, is discussed.

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References

Galzigna L, Rossi C, SARTORELLI L, Gibson D . A guanosine triphosphate-dependent acyl coenzyme A synthetase from rat liver mitochondria. J Biol Chem. 1967; 242(9):2111-5. View

Zammit V . Mechanisms of regulation of the partition of fatty acids between oxidation and esterification in the liver. Prog Lipid Res. 1984; 23(1):39-67. DOI: 10.1016/0163-7827(84)90005-5. View

Ohlendieck K, Riesinger I, Adams V, Krause J, Brdiczka D . Enrichment and biochemical characterization of boundary membrane contact sites from rat-liver mitochondria. Biochim Biophys Acta. 1986; 860(3):672-89. DOI: 10.1016/0005-2736(86)90567-5. View

Declercq P, Falck J, Kuwajima M, Tyminski H, Foster D, McGarry J . Characterization of the mitochondrial carnitine palmitoyltransferase enzyme system. I. Use of inhibitors. J Biol Chem. 1987; 262(20):9812-21. View

Halestrap A . The regulation of the matrix volume of mammalian mitochondria in vivo and in vitro and its role in the control of mitochondrial metabolism. Biochim Biophys Acta. 1989; 973(3):355-82. DOI: 10.1016/s0005-2728(89)80378-0. View

Adams V, Bosch W, Schlegel J, Wallimann T, Brdiczka D . Further characterization of contact sites from mitochondria of different tissues: topology of peripheral kinases. Biochim Biophys Acta. 1989; 981(2):213-25. DOI: 10.1016/0005-2736(89)90031-x. View

Kolodziej M, Zammit V . Re-evaluation of the interaction of malonyl-CoA with the rat liver mitochondrial carnitine palmitoyltransferase system by using purified outer membranes. Biochem J. 1990; 267(1):85-90. PMC: 1131248. DOI: 10.1042/bj2670085. View

Vance J . Phospholipid synthesis in a membrane fraction associated with mitochondria. J Biol Chem. 1990; 265(13):7248-56. View

Skerjanc I, Sheffield W, Randall S, Silvius J, Shore G . Import of precursor proteins into mitochondria: site of polypeptide unfolding. J Biol Chem. 1990; 265(16):9444-51. View

10.

Kerner J, Bieber L . Isolation of a malonyl-CoA-sensitive CPT/beta-oxidation enzyme complex from heart mitochondria. Biochemistry. 1990; 29(18):4326-34. DOI: 10.1021/bi00470a010. View

11.

Woeltje K, ESSER V, Weis B, Sen A, COX W, McPhaul M . Cloning, sequencing, and expression of a cDNA encoding rat liver mitochondrial carnitine palmitoyltransferase II. J Biol Chem. 1990; 265(18):10720-5. View

12.

Biermans W, Bakker A, Jacob W . Contact site between inner and outer mitochondrial membrane: a dynamic microcompartment for creatine kinase activity. Biochim Biophys Acta. 1990; 1018(2-3):225-8. DOI: 10.1016/0005-2728(90)90254-2. View

13.

Brdiczka D, Bucheler K, Kottke M, Adams V, Nalam V . Characterization and metabolic function of mitochondrial contact sites. Biochim Biophys Acta. 1990; 1018(2-3):234-8. DOI: 10.1016/0005-2728(90)90256-4. View

14.

Pfanner N, Rassow J, Wienhues U, Hergersberg C, Sollner T, Becker K . Contact sites between inner and outer membranes: structure and role in protein translocation into the mitochondria. Biochim Biophys Acta. 1990; 1018(2-3):239-42. DOI: 10.1016/0005-2728(90)90257-5. View

15.

Ardail D, Privat J, Levrat C, Lerme F, Louisot P . Mitochondrial contact sites. Lipid composition and dynamics. J Biol Chem. 1990; 265(31):18797-802. View

16.

Kolodziej M, Zammit V . Sensitivity of inhibition of rat liver mitochondrial outer-membrane carnitine palmitoyltransferase by malonyl-CoA to chemical- and temperature-induced changes in membrane fluidity. Biochem J. 1990; 272(2):421-5. PMC: 1149716. DOI: 10.1042/bj2720421. View

17.

Bucheler K, Adams V, Brdiczka D . Localization of the ATP/ADP translocator in the inner membrane and regulation of contact sites between mitochondrial envelope membranes by ADP. A study on freeze-fractured isolated liver mitochondria. Biochim Biophys Acta. 1991; 1056(3):233-42. DOI: 10.1016/s0005-2728(05)80054-4. View

18.

Ardail D, Lerme F, Louisot P . Involvement of contact sites in phosphatidylserine import into liver mitochondria. J Biol Chem. 1991; 266(13):7978-81. View

19.

Brdiczka D . Contact sites between mitochondrial envelope membranes. Structure and function in energy- and protein-transfer. Biochim Biophys Acta. 1991; 1071(3):291-312. DOI: 10.1016/0304-4157(91)90018-r. View

20.

Kolodziej M, Crilly P, Corstorphine C, Zammit V . Development and characterization of a polyclonal antibody against rat liver mitochondrial overt carnitine palmitoyltransferase (CPT I). Distinction of CPT I from CPT II and of isoforms of CPT I in different tissues. Biochem J. 1992; 282 ( Pt 2):415-21. PMC: 1130794. DOI: 10.1042/bj2820415. View