Molecular Characterization of an Outer Membrane Protein of Actinobacillus Actinomycetemcomitans Belonging to the OmpA Family

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1998 Jan 10

PMID 9423883

Citations 6

Authors

P A White

S P Nair

M J Kim

M Wilson

B Henderson

Affiliations

Soon will be listed here.

Abstract

The major outer membrane protein (OMP) of Actinobacillus actinomycetemcomitans is an OmpA homolog that demonstrates electrophoretic heat modifiability. The gene encoding this protein was isolated from a genomic library of A. actinomycetemcomitans NCTC 9710 by immunoscreening with serum from a patient with localized juvenile periodontitis. Expression of the cloned gene in Escherichia coli and subsequent Western blot analysis revealed a protein with an approximate molecular mass of 34 kDa. The amino acid sequence predicted from the cloned gene demonstrated that the mature protein had a molecular mass of 34,911 Da and significant identity to members of the OmpA family of proteins. We have named the major OMP of A. actinomycetemcomitans Omp34, and its corresponding gene has been named omp34.

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References

Marandi M, Mittal K . Characterization of an outer membrane protein of Pasteurella multocida belonging to the OmpA family. Vet Microbiol. 1996; 53(3-4):303-14. DOI: 10.1016/s0378-1135(96)01219-9. View

Reddi K, Nair S, White P, Hodges S, Tabona P, Meghji S . Surface-associated material from the bacterium Actinobacillus actinomycetemcomitans contains a peptide which, in contrast to lipopolysaccharide, directly stimulates fibroblast interleukin-6 gene transcription. Eur J Biochem. 1996; 236(3):871-6. DOI: 10.1111/j.1432-1033.1996.00871.x. View

Costello G, Vipond R, Macintyre S . Aeromonas salmonicida possesses two genes encoding homologs of the major outer membrane protein, OmpA. J Bacteriol. 1996; 178(6):1623-30. PMC: 177847. DOI: 10.1128/jb.178.6.1623-1630.1996. View

Wilson M, Henderson B . Virulence factors of Actinobacillus actinomycetemcomitans relevant to the pathogenesis of inflammatory periodontal diseases. FEMS Microbiol Rev. 1995; 17(4):365-79. DOI: 10.1111/j.1574-6976.1995.tb00220.x. View

Prasadarao N, Wass C, Weiser J, Stins M, Huang S, Kim K . Outer membrane protein A of Escherichia coli contributes to invasion of brain microvascular endothelial cells. Infect Immun. 1996; 64(1):146-53. PMC: 173739. DOI: 10.1128/iai.64.1.146-153.1996. View

Reddi K, Wilson M, Poole S, Meghji S, Henderson B . Relative cytokine-stimulating activities of surface components of the oral periodontopathogenic bacterium Actinobacillus actinomycetemcomitans. Cytokine. 1995; 7(6):534-41. DOI: 10.1006/cyto.1995.0072. View

Koebnik R . Proposal for a peptidoglycan-associating alpha-helical motif in the C-terminal regions of some bacterial cell-surface proteins. Mol Microbiol. 1995; 16(6):1269-70. DOI: 10.1111/j.1365-2958.1995.tb02348.x. View

Kirby A, Meghji S, Nair S, White P, Reddi K, Nishihara T . The potent bone-resorbing mediator of Actinobacillus actinomycetemcomitans is homologous to the molecular chaperone GroEL. J Clin Invest. 1995; 96(3):1185-94. PMC: 185737. DOI: 10.1172/JCI118150. View

White P, Wilson M, Nair S, Kirby A, Reddi K, Henderson B . Characterization of an antiproliferative surface-associated protein from Actinobacillus actinomycetemcomitans which can be neutralized by sera from a proportion of patients with localized juvenile periodontitis. Infect Immun. 1995; 63(7):2612-8. PMC: 173350. DOI: 10.1128/iai.63.7.2612-2618.1995. View

10.

Meghji S, Henderson B, Kirby A, Newman H, Wilson M . Serum antibody response to surface-associated material from periodontopathogenic bacteria. FEMS Immunol Med Microbiol. 1995; 10(2):101-8. DOI: 10.1111/j.1574-695X.1995.tb00017.x. View

11.

Ebersole J, Cappelli D . Gingival crevicular fluid antibody to Actinobacillus actinomycetemcomitans in periodontal disease. Oral Microbiol Immunol. 1994; 9(6):335-44. DOI: 10.1111/j.1399-302x.1994.tb00283.x. View

12.

Wilson M, Hamilton R . Immunoglobulin G subclass response of juvenile periodontitis subjects to principal outer membrane proteins of Actinobacillus actinomycetemcomitans. Infect Immun. 1995; 63(3):1062-9. PMC: 173110. DOI: 10.1128/iai.63.3.1062-1069.1995. View

13.

Mintz K . Identification of an immunoglobulin Fc receptor of Actinobacillus actinomycetemcomitans. Infect Immun. 1994; 62(10):4500-5. PMC: 303136. DOI: 10.1128/iai.62.10.4500-4505.1994. View

14.

De Mot R, Vanderleyden J . The C-terminal sequence conservation between OmpA-related outer membrane proteins and MotB suggests a common function in both gram-positive and gram-negative bacteria, possibly in the interaction of these domains with peptidoglycan. Mol Microbiol. 1994; 12(2):333-4. DOI: 10.1111/j.1365-2958.1994.tb01021.x. View

15.

Saito A, Hosaka Y, Nakagawa T, Seida K, Yamada S, TAKAZOE I . Significance of serum antibody against surface antigens of Actinobacillus actinomycetemcomitans in patients with adult periodontitis. Oral Microbiol Immunol. 1993; 8(3):146-53. DOI: 10.1111/j.1399-302x.1993.tb00657.x. View

16.

Munson Jr R, Grass S, West R . Molecular cloning and sequence of the gene for outer membrane protein P5 of Haemophilus influenzae. Infect Immun. 1993; 61(9):4017-20. PMC: 281112. DOI: 10.1128/iai.61.9.4017-4020.1993. View

17.

Meghji S, Henderson B, Wilson M . Higher-titer antisera from patients with periodontal disease inhibit bacterial capsule-induced bone breakdown. J Periodontal Res. 1993; 28(2):115-21. DOI: 10.1111/j.1600-0765.1993.tb01058.x. View

18.

TOLO K, Helgeland K . Fc-binding components: a virulence factor in Actinobacillus actinomycetemcomitans?. Oral Microbiol Immunol. 1991; 6(6):373-7. DOI: 10.1111/j.1399-302x.1991.tb00509.x. View

19.

Higgins D, Bleasby A, Fuchs R . CLUSTAL V: improved software for multiple sequence alignment. Comput Appl Biosci. 1992; 8(2):189-91. DOI: 10.1093/bioinformatics/8.2.189. View

20.

Dear S, Staden R . A sequence assembly and editing program for efficient management of large projects. Nucleic Acids Res. 1991; 19(14):3907-11. PMC: 328482. DOI: 10.1093/nar/19.14.3907. View