ATP-dependent Desensitization of the Muscarinic K+ Channel in Rat Atrial Cells

Overview

Journal J Physiol

Specialty Physiology

Date 1997 Dec 31

PMID 9409473

Citations 5

Authors

Z Shui

M R Boyett

W J Zang

Affiliations

Soon will be listed here.

Abstract

1. Fast desensitization of the muscarinic K+ channel has been studied in excised patches from rat atrial cells. 2. In inside-out patches, ACh was present in the pipette and GTP was applied via the bath to activate the channel. In outside-out patches, GTP was present in the pipette and ACh was applied via the bath to activate the channel. In both cases, during a 30 s exposure to GTP or ACh there was a decline in channel activity as a result of fast desensitization if ATP was present. 3. In inside-out patches, fast desensitization was still observed if the muscarinic ACh receptor was bypassed and the channel was activated by GTP gamma S. This suggests that fast desensitization is a result of a modification of the channel (or the connecting G protein) and not the receptor. 4. In both inside-out and outside-out patches, channel activity was depressed and fast desensitization was reduced or absent, if ATP was not present. 5. The non-hydrolysable analogue of ATP, AMP-PNP, did not substitute for ATP in its effects on the channel. 6. The results are consistent with the hypothesis that fast desensitization of the muscarinic K+ channel is the result of a dephosphorylation of the channel.

Citing Articles

Short-term desensitization of muscarinic K+ current in the heart.

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Receptor-induced depletion of phosphatidylinositol 4,5-bisphosphate inhibits inwardly rectifying K+ channels in a receptor-specific manner.

Cho H, Lee D, Lee S, Ho W Proc Natl Acad Sci U S A. 2005; 102(12):4643-8.

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Acute desensitization of GIRK current in rat atrial myocytes is related to K+ current flow.

Bender K, Wellner-Kienitz M, Bosche L, Rinne A, Beckmann C, Pott L J Physiol. 2004; 561(Pt 2):471-83.

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Evidence that the nucleotide exchange and hydrolysis cycle of G proteins causes acute desensitization of G-protein gated inward rectifier K+ channels.

Chuang H, Yu M, Jan Y, Jan L Proc Natl Acad Sci U S A. 1998; 95(20):11727-32.

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Role of receptor kinase in short-term desensitization of cardiac muscarinic K+ channels expressed in Chinese hamster ovary cells.

Shui Z, Khan I, Tsuga H, Haga T, Boyett M J Physiol. 1998; 507 ( Pt 2):325-34.

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