The Effect of Some Glucocorticoids on Hepatic Microsomal Hydroxylation in the Rat and Hamster

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1976 Mar 16

PMID 940604

Authors

J M Tredger

F J McPherson

J Chakraborty

J W Bridges

D V Parke

Affiliations

Soon will be listed here.

Abstract

The effect of some glucocorticoids on hepatic microsomal biphenyl and aniline hydroxylations were investigated in rat and hamster. Steroids were either added to the standard incubation mixture or given as a single dose intraperitoneally before preparation of the liver microsomes for enzyme determination. The addition of steroids in vitro enhanced biphenyl-2-hydroxylation activity in hepatic microsomes of rat but not of hamster and the most pronounced effect was obtained with betamethasone. A similar species difference in the effects of betamethasone on this enzyme was also observed after administration of a single dose of the steroid in vivo.

References

Tephly T, MANNERING G . Inhibition of drug metabolism. V. Inhibition of drug metabolism by steroids. Mol Pharmacol. 1968; 4(1):10-4. View

Burke M, Bridges J, Parke D . The effects of the non-ionic detergent Tween 80 on hepatic microsomal hydroxylation. Xenobiotica. 1975; 5(5):261-77. DOI: 10.3109/00498257509052061. View

Wada F, Shimakawa H, Takasugi M, Kotake T, Sakamoto Y . Effects of steroid hormones on drug-metabolizing enzyme systems in liver microsomes. J Biochem. 1968; 64(1):109-13. DOI: 10.1093/oxfordjournals.jbchem.a128854. View

Omura T, Sato R . THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE. J Biol Chem. 1964; 239:2370-8. View

Guarino A, Gram T, Gigon P, Greene F, GILLETTE J . Changes in Michaelis and spectral constants for aniline in hepatic microsomes from phenobarbital-treated rats. Mol Pharmacol. 1969; 5(2):131-6. View

Lewis D, Symons A, Ancill R . The stabilization-lysis action of anti-inflammatory steroids on lysosomes. J Pharm Pharmacol. 1970; 22(12):902-8. DOI: 10.1111/j.2042-7158.1970.tb08471.x. View

Kuntzman R, Jacobson M, SCHNEIDMAN K, Conney A . SIMILARITIES BETWEEN OXIDATIVE DRUG-METABOLIZING ENZYMES AND STEROID HYDROXYLASES IN LIVER MICROSOMES. J Pharmacol Exp Ther. 1964; 146:280-5. View

Creaven P, Parke D, Williams R . A fluorimetric study of the hydroxylation of biphenyl in vitro by liver preparations of various species. Biochem J. 1965; 96(3):879-85. PMC: 1207231. DOI: 10.1042/bj0960879. View

Creaven P, Parke D . The stimulation of hydroxylation by carcinogenic and non-carcinogenic compounds. Biochem Pharmacol. 1966; 15(1):7-18. DOI: 10.1016/0006-2952(66)90105-5. View

10.

Bangham A, Standish M, Weissmann G . The action of steroids and streptolysin S on the permeability of phospholipid structures to cations. J Mol Biol. 1965; 13(1):253-9. DOI: 10.1016/s0022-2836(65)80094-8. View

11.

McPHERSON F, Bridges J, Parke D . In vitro enhancement of hepatic microsomal biphenyl 2-hydroxylation by carcinogens. Nature. 1974; 252(5483):488-9. DOI: 10.1038/252488a0. View

12.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

13.

Briggs M . Lysosomal enzyme activation by steroid hormones in vivo. J Steroid Biochem. 1973; 4(4):341-7. DOI: 10.1016/0022-4731(73)90003-4. View

14.

Rabin B, Blyth C, Doherty D, Freedman R, Roobol A, Sunshine G . The neoplastic cell. The effects of steroid hormones and carcinogens on the interaction of membranes with polysomes. J Clin Pathol Suppl (R Coll Pathol). 1974; 7:51-9. PMC: 1347227. View