Molecular Typing of Acinetobacter Baumannii from Ten Different Intensive Care Units of a University Hospital

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 1997 Dec 24

PMID 9405944

Citations 2

Authors

E Presterl

R Nadrchal

S Winkler

A Makristathis

W Koller

M L Rotter

A M Hirschl

Affiliations

Soon will be listed here.

Abstract

Thirty-one isolates of Acinetobacter baumannii were collected from ten intensive care units of an Austrian university hospital. All isolates were typed by enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Two strains colonizing 13 infants in the neonatal intensive care unit were identified by ERIC-PCR. All other Acinetobacter baumannii isolates had highly divergent ERIC-PCR patterns, despite having the same antibiogram. Thus, a hospital-wide clonal distribution, as suggested by identical antibiogram patterns, was excluded by ERIC-PCR.

Citing Articles

Control of an Acinetobacter [corrected] baumannii outbreak in a neonatal ICU without suspension of service: a devastating outbreak in Diyarbakir, Turkey.

Hosoglu S, Hascuhadar M, Yasar E, Uslu S, Aldudak B Infection. 2011; 40(1):11-8.

PMID: 21881956 DOI: 10.1007/s15010-011-0180-y.

Molecular characterization of hand flora and environmental isolates in a community setting.

Pancholi P, Healy M, Bittner T, Webb R, Wu F, Aiello A J Clin Microbiol. 2005; 43(10):5202-7.

PMID: 16207984 PMC: 1248454. DOI: 10.1128/JCM.43.10.5202-5207.2005.

References

Bergogne-Berezin E . The increasing significance of outbreaks of Acinetobacter spp.: the need for control and new agents. J Hosp Infect. 1995; 30 Suppl:441-52. DOI: 10.1016/0195-6701(95)90048-9. View

Snelling A, Gerner-Smidt P, Hawkey P, Heritage J, Parnell P, Porter C . Validation of use of whole-cell repetitive extragenic palindromic sequence-based PCR (REP-PCR) for typing strains belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii complex and application of the method to the investigation of a.... J Clin Microbiol. 1996; 34(5):1193-202. PMC: 228981. DOI: 10.1128/jcm.34.5.1193-1202.1996. View

Versalovic J, Koeuth T, Lupski J . Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991; 19(24):6823-31. PMC: 329316. DOI: 10.1093/nar/19.24.6823. View

Lyytikainen O, Koljalg S, Harma M, Vuopio-Varkila J . Outbreak caused by two multi-resistant Acinetobacter baumannii clones in a burns unit: emergence of resistance to imipenem. J Hosp Infect. 1995; 31(1):41-54. DOI: 10.1016/0195-6701(95)90082-9. View

Dijkshoorn L, Aucken H, Gerner-Smidt P, Janssen P, Kaufmann M, Garaizar J . Comparison of outbreak and nonoutbreak Acinetobacter baumannii strains by genotypic and phenotypic methods. J Clin Microbiol. 1996; 34(6):1519-25. PMC: 229053. DOI: 10.1128/jcm.34.6.1519-1525.1996. View

Bergogne-Berezin E, Towner K . Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev. 1996; 9(2):148-65. PMC: 172888. DOI: 10.1128/CMR.9.2.148. View

Reboli A, Houston E, Monteforte J, Wood C, Hamill R . Discrimination of epidemic and sporadic isolates of Acinetobacter baumannii by repetitive element PCR-mediated DNA fingerprinting. J Clin Microbiol. 1994; 32(11):2635-40. PMC: 264134. DOI: 10.1128/jcm.32.11.2635-2640.1994. View

Marcos M, Jimenez de Anta M, Vila J . Correlation of six methods for typing nosocomial isolates of Acinetobacter baumannii. J Med Microbiol. 1995; 42(5):328-35. DOI: 10.1099/00222615-42-5-328. View

Struelens M, Carlier E, Maes N, Serruys E, Quint W, van Belkum A . Nosocomial colonization and infection with multiresistant Acinetobacter baumannii: outbreak delineation using DNA macrorestriction analysis and PCR-fingerprinting. J Hosp Infect. 1993; 25(1):15-32. DOI: 10.1016/0195-6701(93)90005-k. View

10.

Liu P, Lau Y, Hu B, Shyr J, Shi Z, Tsai W . Analysis of clonal relationships among isolates of Shigella sonnei by different molecular typing methods. J Clin Microbiol. 1995; 33(7):1779-83. PMC: 228268. DOI: 10.1128/jcm.33.7.1779-1783.1995. View

11.

Levin A, Mendes C, Sinto S, Sader H, Scarpitta C, Rodrigues E . An outbreak of multiresistant Acinetobacter baumanii in a university hospital in São Paulo, Brazil. Infect Control Hosp Epidemiol. 1996; 17(6):366-8. DOI: 10.1086/647319. View

12.

Gilson E, CLEMENT J, Brutlag D, Hofnung M . A family of dispersed repetitive extragenic palindromic DNA sequences in E. coli. EMBO J. 1984; 3(6):1417-21. PMC: 557532. DOI: 10.1002/j.1460-2075.1984.tb01986.x. View

13.

Ehrenstein B, Bernards A, Dijkshoorn L, Gerner-Smidt P, Towner K, Bouvet P . Acinetobacter species identification by using tRNA spacer fingerprinting. J Clin Microbiol. 1996; 34(10):2414-20. PMC: 229283. DOI: 10.1128/jcm.34.10.2414-2420.1996. View