Ca2+/calmodulin-binding Peptides Block Phototransduction in Limulus Ventral Photoreceptors: Evidence for Direct Inhibition of Phospholipase C

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1998 Feb 12

PMID 9391158

Citations 3

Authors

E A Richard

S Ghosh

J M Lowenstein

J E Lisman

Affiliations

Soon will be listed here.

Abstract

Phototransduction in Limulus photoreceptors involves a G protein-mediated activation of phospholipase C (PLC) and subsequent steps involving InsP3-mediated release of intracellular Ca2+. While exploring the role of calmodulin in this cascade, we found that intracellular injection of Ca2+/calmodulin-binding peptides (CCBPs) strongly inhibited the light response. By chemically exciting the cascade at various stages, we found the primary target of this effect was not in late stages of the cascade but rather at the level of G protein and PLC. That PLCdelta1 contains a calmodulin-like structure raised the possibility that PLC might be directly affected by CCBPs. To test this possibility, in vitro experiments were conducted on purified PLC. The activity of this enzyme was strongly inhibited by CCBPs and also inhibited by calmodulin itself. Our results suggest that the calmodulin-like region of PLC has an important role in regulating this enzyme.

Citing Articles

The excitation cascade of Limulus ventral photoreceptors: guanylate cyclase as the link between InsP3-mediated Ca2+ release and the opening of cGMP-gated channels.

Garger A, Richard E, Lisman J BMC Neurosci. 2004; 5:7.

PMID: 15053840 PMC: 375529. DOI: 10.1186/1471-2202-5-7.

The ordered visual transduction complex of the squid photoreceptor membrane.

Lott J, Wilde J, Carne A, Evans N, Findlay J Mol Neurobiol. 1999; 20(1):61-80.

PMID: 10595873 DOI: 10.1007/BF02741365.

A Ca2+/calmodulin-dependent protein kinase modulates Drosophila photoreceptor K+ currents: a role in shaping the photoreceptor potential.

Peretz A, Abitbol I, Sobko A, Wu C, Attali B J Neurosci. 1998; 18(22):9153-62.

PMID: 9801355 PMC: 6792883.

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