Radiation Damage in Tripalmitin Layers Studied by Means of Infrared Spectroscopy and Electron Microscopy

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1976 Jul 1

PMID 938719

Citations 1

Authors

W Baumeister

U P Fringeli

M Hahn

F Kopp

J Seredynski

Affiliations

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Abstract

Structural deteriorations in biomembranes, as inevitably induced while structural information is gathered by electron optical methods, were evaluated by infrared spectroscopy. Tripalmitin model membranes were irradiated with 100 keV-electrons in an electron microscope. The intensity decay of group vibrations over the dose reveals the sequence of damage in the polar and nonpolar part of the molecule. The C-C backbone, being the most important structural feature, shows a significant latency effect up to 0.6 e-/A2 and is completely disordered by 3 e-/A2, corresponding to about three inelastic processes per molecule.

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Baumeister W Protein Sci. 2004; 14(1):257-69.

PMID: 15608126 PMC: 2253336. DOI: 10.1110/ps.041148605.

References

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