Proteoglycan Synthesis in Haematopoietic Cells: Isolation and Characterization of Heparan Sulphate Proteoglycans Expressed by the Bone-marrow Stromal Cell Line MS-5

Overview

Journal Biochem J

Specialty Biochemistry

Date 1997 Nov 14

PMID 9359418

Citations 11

Authors

Z Drzeniek

B Siebertz

G Stocker

U Just

W Ostertag

H Greiling

H D Haubeck

Affiliations

Soon will be listed here.

Abstract

Proteoglycans of bone-marrow stromal cells and their extracellular matrix are important components of the haematopoietic microenvironment. Recently, several studies have indicated that they are involved in the interaction of haematopoietic stem and stromal cells. However, a detailed characterization of the heparan sulphate proteoglycans synthesized by bone-marrow stromal cells is still lacking. Here we report on the isolation and characterization of proteoglycans from the haematopoietic stromal cell line MS-5, that efficiently supports the growth and differentiation of human and murine haematopoietic progenitor cells. Biochemical characterization of purified proteoglycans revealed that the haematopoietic stromal cell line MS-5 synthesizes, in addition to chondroitin sulphate proteoglycans, several different heparan sulphate proteoglycans. Immunochemical analysis, using specific antibodies against the different members of the syndecan family, glypican, betaglycan and perlecan, showed that MS-5 cells synthesize all these different heparan sulphate proteoglycans. These data were further supported by reverse-transcriptase PCR and confirmed by sequence and Northern blot analysis. The relative abundance of the different heparan sulphate proteoglycans was estimated on the protein and mRNA levels.

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References

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Rapraeger A, Krufka A, Olwin B . Requirement of heparan sulfate for bFGF-mediated fibroblast growth and myoblast differentiation. Science. 1991; 252(5013):1705-8. DOI: 10.1126/science.1646484. View

Dexter T . Stromal cell associated haemopoiesis. J Cell Physiol Suppl. 1982; 1:87-94. DOI: 10.1002/jcp.1041130414. View

Gallagher J, Spooncer E, Dexter T . Role of the cellular matrix in haemopoiesis. I. Synthesis of glycosaminoglycans by mouse bone marrow cell cultures. J Cell Sci. 1983; 63:155-71. DOI: 10.1242/jcs.63.1.155. View

Heinegard D, Coster L, Franzen A, GARDELL S, Malmstrom A, Paulsson M . The core proteins of large and small interstitial proteoglycans from various connective tissues form distinct subgroups. Biochem J. 1985; 230(1):181-94. PMC: 1152601. DOI: 10.1042/bj2300181. View

Kato M, Oike Y, Suzuki S, Kimata K . Selective removal of heparan sulfate chains from proteoheparan sulfate with a commercial preparation of heparitinase. Anal Biochem. 1985; 148(2):479-84. DOI: 10.1016/0003-2697(85)90255-6. View

Wight T, Kinsella M, Keating A, Singer J . Proteoglycans in human long-term bone marrow cultures: biochemical and ultrastructural analyses. Blood. 1986; 67(5):1333-43. View

Spooncer E, Heyworth C, Dunn A, Dexter T . Self-renewal and differentiation of interleukin-3-dependent multipotent stem cells are modulated by stromal cells and serum factors. Differentiation. 1986; 31(2):111-8. DOI: 10.1111/j.1432-0436.1986.tb00391.x. View

Morris A, Turnbull J, Riley G, Gordon M, Gallagher J . Production of heparan sulphate proteoglycans by human bone marrow stromal cells. J Cell Sci. 1991; 99 ( Pt 1):149-56. DOI: 10.1242/jcs.99.1.149. View

10.

Khorana H . Rhodopsin, photoreceptor of the rod cell. An emerging pattern for structure and function. J Biol Chem. 1992; 267(1):1-4. View

11.

Lories V, Cassiman J, Van Den Berghe H, David G . Differential expression of cell surface heparan sulfate proteoglycans in human mammary epithelial cells and lung fibroblasts. J Biol Chem. 1992; 267(2):1116-22. View

12.

Murdoch A, Dodge G, Cohen I, Tuan R, Iozzo R . Primary structure of the human heparan sulfate proteoglycan from basement membrane (HSPG2/perlecan). A chimeric molecule with multiple domains homologous to the low density lipoprotein receptor, laminin, neural cell adhesion molecules, and epidermal.... J Biol Chem. 1992; 267(12):8544-57. View

13.

Yanagishita M, Hascall V . Cell surface heparan sulfate proteoglycans. J Biol Chem. 1992; 267(14):9451-4. View

14.

Turnbull J, Fernig D, Ke Y, Wilkinson M, Gallagher J . Identification of the basic fibroblast growth factor binding sequence in fibroblast heparan sulfate. J Biol Chem. 1992; 267(15):10337-41. View

15.

Minguell J, Hardy C, Tavassoli M . Membrane-associated chondroitin sulfate proteoglycan and fibronectin mediate the binding of hemopoietic progenitor cells to stromal cells. Exp Cell Res. 1992; 201(1):200-7. DOI: 10.1016/0014-4827(92)90364-e. View

16.

Shirota T, Minguell J, Tavassoli M . Expression of chondroitin sulfate as a unique type of proteoglycan on the cell membrane of multipotential and committed hemopoietic progenitor cells. Biochim Biophys Acta. 1992; 1136(1):17-22. DOI: 10.1016/0167-4889(92)90079-q. View

17.

Vainio S, Jalkanen M, Bernfield M, SAXEN L . Transient expression of syndecan in mesenchymal cell aggregates of the embryonic kidney. Dev Biol. 1992; 152(2):221-32. DOI: 10.1016/0012-1606(92)90130-9. View

18.

Siczkowski M, Clarke D, Gordon M . Binding of primitive hematopoietic progenitor cells to marrow stromal cells involves heparan sulfate. Blood. 1992; 80(4):912-9. View

19.

David G, Van der Schueren B, Marynen P, Cassiman J, Van Den Berghe H . Molecular cloning of amphiglycan, a novel integral membrane heparan sulfate proteoglycan expressed by epithelial and fibroblastic cells. J Cell Biol. 1992; 118(4):961-9. PMC: 2289559. DOI: 10.1083/jcb.118.4.961. View

20.

Sanderson R, Hinkes M, Bernfield M . Syndecan-1, a cell-surface proteoglycan, changes in size and abundance when keratinocytes stratify. J Invest Dermatol. 1992; 99(4):390-6. DOI: 10.1111/1523-1747.ep12616103. View