Alpha 2.0
Login Register
» Articles » PMID: 9350723

Molecular Diagnosis of Bacterial Endocarditis by Broad-range PCR Amplification and Direct Sequencing

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1997 Nov 14
PMID 9350723
Citations 122
Authors
D Goldenberger
A Kunzli
P Vogt
R Zbinden
M Altwegg
Affiliations
Soon will be listed here.
Abstract

Broad-range PCR amplification of part of the 16S rRNA gene followed by single-strand sequencing was applied to samples of 18 resected heart valves from patients with infective endocarditis. The PCR results were compared with those of cultures of valves and with those of previous blood cultures. For two patients there was agreement with the cultures of the valves; for nine patients there was agreement with the previous blood cultures, which were positive, while the cultures of the valves were negative; a Streptococcus sp. and Tropheryma whippelii each were found in one patient with negative cultures (valve and blood); for two patients the cultures of the valves as well as the PCR results were negative but the blood cultures were positive; for one patient amplification was inhibited; and for two patients the PCR results were positive but the amplicons could not be sequenced. It is concluded that broad-range PCR is a promising tool for patients with culture-negative endocarditis and allows the detection of rare, noncultivable organisms.

Citing Articles

The expanding role of 16s ribosomal RNA PCR in the management of patients with infective endocarditis undergoing cardiac surgery.

Pavone N, Cammertoni F, Calabrese M, Bruno P, Scoppettuolo G, Lombardo A Front Cardiovasc Med. 2025; 11:1504197.

PMID: 39744206 PMC: 11688630. DOI: 10.3389/fcvm.2024.1504197.

Intraoperative Polymerase Chain Reaction from Cardiac Valve Tissue Is Beneficial for Guiding Further Therapy in Patients with Infective Endocarditis.

von Zeppelin M, Gharoony S, Holubcova Z, Salem R, Hlavicka J, Heyl S J Clin Med. 2024; 13(15).

PMID: 39124586 PMC: 11313656. DOI: 10.3390/jcm13154319.

Uncommon pathogens causing infective endocarditis.

Menon T Indian J Thorac Cardiovasc Surg. 2024; 40(Suppl 1):8-15.

PMID: 38827550 PMC: 11139812. DOI: 10.1007/s12055-023-01627-z.

[Endocarditis and ischemic stroke of rare cause].

Schonfeld L, Scheumann G, Jockel J Inn Med (Heidelb). 2024; 65(8):840-842.

PMID: 38483553 DOI: 10.1007/s00108-024-01685-4.

16S rRNA Gene PCR/Sequencing of Heart Valves for Diagnosis of Infective Endocarditis in Routine Clinical Practice.

Hong H, Flurin L, Greenwood-Quaintance K, Wolf M, Pritt B, Norgan A J Clin Microbiol. 2023; 61(8):e0034123.

PMID: 37436146 PMC: 10446860. DOI: 10.1128/jcm.00341-23.

References
1.
Kawamura Y, Hou X, Sultana F, Miura H, Ezaki T . Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus. Int J Syst Bacteriol. 1995; 45(2):406-8. DOI: 10.1099/00207713-45-2-406. View
2.
Goldenberger D, Schmidheini T, Altwegg M . Detection of Bartonella henselae and Bartonella quintana by a simple and rapid procedure using broad-range PCR amplification and direct single-strand sequencing of part of the 16S rRNA gene. Clin Microbiol Infect. 1997; 3(2):240-245. DOI: 10.1111/j.1469-0691.1997.tb00604.x. View
3.
Frothingham R, WILSON K . Molecular phylogeny of the Mycobacterium avium complex demonstrates clinically meaningful divisions. J Infect Dis. 1994; 169(2):305-12. DOI: 10.1093/infdis/169.2.305. View
4.
Wendler D, Mendoza E, Schleiffer T, Zander M, Maier M . Tropheryma whippelii endocarditis confirmed by polymerase chain reaction. Eur Heart J. 1995; 16(3):424-5. DOI: 10.1093/oxfordjournals.eurheartj.a060928. View
5.
Carbon P, Ehresmann C, Ehresmann B, Ebel J . The complete nucleotide sequence of the ribosomal 16-S RNA from Excherichia coli. Experimental details and cistron heterogeneities. Eur J Biochem. 1979; 100(2):399-410. DOI: 10.1111/j.1432-1033.1979.tb04183.x. View