Studies of Coat Protein-mediated Resistance to Tobacco Mosaic Tobamovirus: Correlation Between Assembly of Mutant Coat Proteins and Resistance

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1997 Oct 6

PMID 9311885

Citations 24

Authors

M Bendahmane

J H Fitchen

G Zhang

R N Beachy

Affiliations

Soon will be listed here.

Abstract

Coat protein-mediated resistance (CP-MR) has been widely used to protect transgenic plants against virus diseases. To characterize the mechanisms of CP-MR to tobacco mosaic tobamovirus (TMV) we developed mutants of the coat protein that affected subunit-subunit interactions. Mutant CPs were expressed during TMV replication as well as in transgenic Nicotiana tabacum plants. The mutation T42-->W increased protein aggregation and T28-->W abolished aggregation and assembly, while the mutations T28-->W plus T42-->W and T89-->W altered normal CP subunit-subunit interactions. The mutant T28W was unable to assemble virus-like particles (VLPs) during infection and in transgenic plants failed to aggregate; this protein conferred no protection against challenge of transgenic plants by TMV. The mutant T42W had strong CP subunit-subunit interactions and formed VLPs but not infectious virions. Transgenic lines with this protein exhibited stronger protection against TMV infection than transgenic plants that contained the wild-type (wt) CP. It is proposed that increased resistance conferred by the T42W mutant results from strong interaction between transgenic CP subunits and challenge virus CP subunits. CP carrying the mutation T89-->W formed flexuous and unstable VLPs whereas the double mutant T28W:T42W formed open helical structures that accumulated as paracrystalline arrays. In transgenic plants, T89W and the double mutant CPs showed reduced ability to aggregate and provided lower protection against TMV infection than wt CP. A strong correlation between normal CP subunit-subunit interactions and CP-MR is observed, and a model for CP-MR involving interactions between the transgenic CP and the CP of the challenge virus as well as interference with virus movement is discussed.

Citing Articles

Conformational behavior of coat protein in plants and association with coat protein-mediated resistance against TMV.

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The Matrix Protein of a Plant Rhabdovirus Mediates Superinfection Exclusion by Inhibiting Viral Transcription.

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Full sequence of the coat protein gene is required for the induction of pathogen-derived resistance against sugarcane mosaic virus in transgenic sugarcane.

Apriasti R, Widyaningrum S, Hidayati W, Sawitri W, Darsono N, Hase T Mol Biol Rep. 2018; 45(6):2749-2758.

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Localization of Movement Protein Modulating Programmed Cell Death in .

Ju J, Kim K, Lee K, Lee W, Ju H Plant Pathol J. 2017; 33(1):53-65.

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The Tomato yellow leaf curl virus V2 protein forms aggregates depending on the cytoskeleton integrity and binds viral genomic DNA.

Moshe A, Belausov E, Niehl A, Heinlein M, Czosnek H, Gorovits R Sci Rep. 2015; 5:9967.

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