Non-invasive Quantitative 31P MRS Assay of Mitochondrial Function in Skeletal Muscle in Situ

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 1997 Oct 6

PMID 9309660

Citations 10

Authors

J A Jeneson

R W Wiseman

M J Kushmerick

Affiliations

Soon will be listed here.

Abstract

A method for non-invasive, quantitative 31P NMR spectroscopic investigation of mitochondrial function in human skeletal muscle in situ is described. High time resolution 31P NMR measurements of phosphocreatine, inorganic phosphate and ATP resonances were conducted on human forearm flexor muscle during involuntary twitch contraction at eight different frequencies. Mitochondrial and glyco(geno)lytic ATP synthesis fluxes, and the cytosolic free energy of ATP hydrolysis (delta GP), were calculated at incremental steady-states of energy balance. The covariation of mitochondrial ATP synthesis flux, JPMOP, and delta GP was quasi-linear over the physiological range of free energy values. Curve-fit analysis of the covariation yielded a maximal sustainable JPMOP of 0.24 +/- 0.06 mmol ATP l-1.s-1 and a midpoint potential, (delta GP)0.5, of 58.1 +/- 1.2 kJ/mole in the muscle cells.

Citing Articles

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