RNA Profile and Structural Protein Analysis of Rotaviruses Isolated from Diarrhoeal Calves in India

Two isolates of group A rotaviruses (CR129 and CR156) were isolated from faecal samples of diarrhoeal calves reared in two dairy farms at Hisar (Haryana, India) by using MA-104 cell lines. These isolates were compared with three standard reference bovine rotaviruses, UK, NCDV and B223, to reveal differences, if any, in their genome and protein migration profiles. The migration of RNA segment 4 of CR129 was slower than that of NCDV, but faster than that of UK. Segment 10 of CR156 moved faster than that of the reference viruses. The segments 2 and 3 co-migrated in CR129, but resolved separately in CR156. Five protein bands of size 116-120 KD (VP1), 95 KD (VP2), 90 KD (VP3/VP4), 44 KD (VP6) and 34 KD (VP7) were detected by protein analysis. No significant difference was observed in the protein profile of these two bovine rotavirus isolates by immunoblotting. However, VP1 was of approximately 116 KD size in the two isolates, compared to 120 KD in the reference strains. These findings indicate that these rotaviruses isolated from diarrhoeic Indian calves differed from the 3 reference strains.