Characterization of Scedosporium Prolificans Clinical Isolates by Randomly Amplified Polymorphic DNA Analysis

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1997 Sep 1

PMID 9276400

Citations 7

Authors

R San Millan

G Quindos

J Garaizar

R Salesa

J Guarro

J Ponton

Affiliations

Soon will be listed here.

Abstract

Fingerprinting by randomly amplified polymorphic DNA (RAPD) analysis was used to differentiate Scedosporium prolificans isolates. A total of 59 arbitrary primers were screened with six unrelated S. prolificans isolates, and a panel of 12 primers was selected. The 12 primers were then used to detect DNA polymorphisms among 17 S. prolificans isolates from 11 patients with systemic S. prolificans infections diagnosed in three hospitals located in geographically different areas of Spain. Eight patients were diagnosed with S. prolificans infection in a single institution over a 6-year period, and two other patients were diagnosed with S. prolificans infection in a different hospital over a 1-year period. No single primer allowed for the discrimination of all the isolates from different patients, but this was possible by combining the RAPD patterns from three primers (UBC 701, AB1.08, and AB1.11 or UBC 701, AB1.08, and UBC 707). However, multiple isolates from the same patient were identical. In this study, we also compared a visual method and a computerized method for the analysis of the RAPD patterns. Both methods were satisfactory and gave few discordances, but given the advantages and disadvantages of each method, both systems should be used together. RAPD analysis provided a fast and economical means of typing S. prolificans isolates, with a high level of discrimination among unrelated isolates. Typing by RAPD analysis confirmed that the S. prolificans infections were epidemiologically unrelated.

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References

Kersters K, De Ley J . Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns. J Gen Microbiol. 1975; 87(2):333-42. DOI: 10.1099/00221287-87-2-333. View

Nenoff P, Gutz U, Tintelnot K, Bosse-Henck A, Mierzwa M, Hofmann J . Disseminated mycosis due to Scedosporium prolificans in an AIDS patient with Burkitt lymphoma. Mycoses. 1996; 39(11-12):461-5. DOI: 10.1111/j.1439-0507.1996.tb00098.x. View

Summerbell R, Krajden S, Kane J . Potted plants in hospitals as reservoirs of pathogenic fungi. Mycopathologia. 1989; 106(1):13-22. DOI: 10.1007/BF00436921. View

Wilson C, ORourke E, McGinnis M, Salkin I . Scedosporium inflatum: clinical spectrum of a newly recognized pathogen. J Infect Dis. 1990; 161(1):102-7. DOI: 10.1093/infdis/161.1.102. View

Howell S, Noble W . Typing tools for the investigation of epidemic fungal infection. Epidemiol Infect. 1990; 105(1):1-9. PMC: 2271802. DOI: 10.1017/s0950268800047580. View

Marin J, Sanz M, Sanz G, Guarro J, Martinez M, Prieto M . Disseminated Scedosporium inflatum infection in a patient with acute myeloblastic leukemia. Eur J Clin Microbiol Infect Dis. 1991; 10(9):759-61. DOI: 10.1007/BF01972505. View

Wood G, McCormack J, Muir D, Ellis D, RIDLEY M, Pritchard R . Clinical features of human infection with Scedosporium inflatum. Clin Infect Dis. 1992; 14(5):1027-33. DOI: 10.1093/clinids/14.5.1027. View

Montenegro E, Fierro F, Fernandez F, Gutierrez S, Martin J . Resolution of chromosomes III and VI of Aspergillus nidulans by pulsed-field gel electrophoresis shows that the penicillin biosynthetic pathway genes pcbAB, pcbC, and penDE are clustered on chromosome VI (3.0 megabases). J Bacteriol. 1992; 174(21):7063-7. PMC: 207390. DOI: 10.1128/jb.174.21.7063-7067.1992. View

Cohen J, Holden D . Use of randomly amplified polymorphic DNA markers to distinguish isolates of Aspergillus fumigatus. J Clin Microbiol. 1992; 30(11):2991-3. PMC: 270566. DOI: 10.1128/jcm.30.11.2991-2993.1992. View

10.

Lehmann P, Lin D, Lasker B . Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA. J Clin Microbiol. 1992; 30(12):3249-54. PMC: 270642. DOI: 10.1128/jcm.30.12.3249-3254.1992. View

11.

Sullivan D, Bennett D, Henman M, Harwood P, Flint S, Mulcahy F . Oligonucleotide fingerprinting of isolates of Candida species other than C. albicans and of atypical Candida species from human immunodeficiency virus-positive and AIDS patients. J Clin Microbiol. 1993; 31(8):2124-33. PMC: 265709. DOI: 10.1128/jcm.31.8.2124-2133.1993. View

12.

Girardin H, Sarfati J, Traore F, Dupouy Camet J, Derouin F, Latge J . Molecular epidemiology of nosocomial invasive aspergillosis. J Clin Microbiol. 1994; 32(3):684-90. PMC: 263107. DOI: 10.1128/jcm.32.3.684-690.1994. View

13.

Tapia M, Richard C, Baro J, Salesa R, Figols J, Zurbano F . Scedosporium inflatum infection in immunocompromised haematological patients. Br J Haematol. 1994; 87(1):212-4. DOI: 10.1111/j.1365-2141.1994.tb04897.x. View

14.

Rabodonirina M, Paulus S, Thevenet F, Loire R, Gueho E, Bastien O . Disseminated Scedosporium prolificans (S. inflatum) infection after single-lung transplantation. Clin Infect Dis. 1994; 19(1):138-42. DOI: 10.1093/clinids/19.1.138. View

15.

Alvarez M, Lopez Ponga B, Rayon C, Gala J, Roson Porto M, Gonzalez M . Nosocomial outbreak caused by Scedosporium prolificans (inflatum): four fatal cases in leukemic patients. J Clin Microbiol. 1995; 33(12):3290-5. PMC: 228690. DOI: 10.1128/jcm.33.12.3290-3295.1995. View

16.

Bouza E, Munoz P, Vega L, Rodriguez-Creixems M, Berenguer J, Escudero A . Clinical resolution of Scedosporium prolificans fungemia associated with reversal of neutropenia following administration of granulocyte colony-stimulating factor. Clin Infect Dis. 1996; 23(1):192-3. DOI: 10.1093/clinids/23.1.192. View

17.

Pickles R, Pacey D, Muir D, Merrell W . Experience with infection by Scedosporium prolificans including apparent cure with fluconazole therapy. J Infect. 1996; 33(3):193-7. DOI: 10.1016/s0163-4453(96)92249-5. View

18.

Power E . RAPD typing in microbiology--a technical review. J Hosp Infect. 1996; 34(4):247-65. DOI: 10.1016/s0195-6701(96)90106-1. View

19.

Tyler K, Wang G, Tyler S, Johnson W . Factors affecting reliability and reproducibility of amplification-based DNA fingerprinting of representative bacterial pathogens. J Clin Microbiol. 1997; 35(2):339-46. PMC: 229576. DOI: 10.1128/jcm.35.2.339-346.1997. View

20.

Salkin I, McGinnis M, Dykstra M, Rinaldi M . Scedosporium inflatum, an emerging pathogen. J Clin Microbiol. 1988; 26(3):498-503. PMC: 266320. DOI: 10.1128/jcm.26.3.498-503.1988. View