Stabilization of Protein Structures

Overview

Journal Curr Opin Biotechnol

Publisher Elsevier

Specialty Biotechnology

Date 1997 Aug 1

PMID 9265720

Citations 14

Authors

B Lee

G Vasmatzis

Affiliations

Soon will be listed here.

Abstract

The technique of protein stabilization has been improving steadily in recent years, but it is only in the past year or two that the stability of some protein molecules has been improved to the level of those from extreme thermophilic organisms. This was achieved by multiple mutations and often by utilizing the knowledge gained from the homologous protein structures from extreme thermophiles.

Citing Articles

Design of Phl p 6 Variants With Altered Fold-Stability Significantly Impacts Antigen Processing, Immunogenicity and Immune Polarization.

Winter P, Stubenvoll S, Scheiblhofer S, Joubert I, Strasser L, Briganser C Front Immunol. 2020; 11:1824.

PMID: 33013833 PMC: 7461793. DOI: 10.3389/fimmu.2020.01824.

Can Predicted Protein 3D Structures Provide Reliable Insights into whether Missense Variants Are Disease Associated?.

Ittisoponpisan S, Islam S, Khanna T, Alhuzimi E, David A, Sternberg M J Mol Biol. 2019; 431(11):2197-2212.

PMID: 30995449 PMC: 6544567. DOI: 10.1016/j.jmb.2019.04.009.

Influence of protein fold stability on immunogenicity and its implications for vaccine design.

Scheiblhofer S, Laimer J, Machado Y, Weiss R, Thalhamer J Expert Rev Vaccines. 2017; 16(5):479-489.

PMID: 28290225 PMC: 5490637. DOI: 10.1080/14760584.2017.1306441.

Improving kinetic or thermodynamic stability of an azoreductase by directed evolution.

Brissos V, Goncalves N, Melo E, Martins L PLoS One. 2014; 9(1):e87209.

PMID: 24475252 PMC: 3903626. DOI: 10.1371/journal.pone.0087209.

The critical role of partially exposed N-terminal valine residue in stabilizing GH10 xylanase from Bacillus sp.NG-27 under poly-extreme conditions.

Bhardwaj A, Bharadwaj A, Leelavathi S, Mazumdar-Leighton S, Ghosh A, Ramakumar S PLoS One. 2008; 3(8):e3063.

PMID: 18725971 PMC: 2516601. DOI: 10.1371/journal.pone.0003063.