Solid-phase Radioimmunoassay for Hepatitis Be Antigen (HBeAg)

Overview

Journal Klin Wochenschr

Specialty General Medicine

Date 1979 Oct 15

PMID 92593

Authors

H E Blum

G Dolken

W Gerok

Affiliations

Soon will be listed here.

Abstract

A solid-phase radioimmunoassay was developed for the detection of HBeAg and anti-HBe in sera or serum fractions. HBe/sAg positive sera, partially purified HBeAg, partially purified HBsAg, and HBe/sAg negative sera were polymerized in polyacrylamide and compared for their ability to bind 125I-IgG (anti-HBe). Only gels containing HBeAg reacted specifically with the iodinated antibody. The specificity of the binding was confirmed by blocking and inhibition tests using anti-HBe, HBeAg, HBsAg, and negative control sera. The radioimmunoassay allows the specific and quantitative detection of HBeAg and anti-HBe even in the presence of detergents and high salt concentrations.

References

Magnius L, Espmark A . A new antigen complex co-occurring with Australia antigen. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972; 80(2):335-7. DOI: 10.1111/j.1699-0463.1972.tb00167.x. View

Krugman S, Overby L, MUSHAHWAR I, Ling C, Frosner G, Deinhardt F . Viral hepatitis, type B. Studies on natural history and prevention re-examined. N Engl J Med. 1979; 300(3):101-6. DOI: 10.1056/NEJM197901183000301. View

Magnius L, ESPMARK J . New specificities in Australia antigen positive sera distinct from the Le Bouvier determinants. J Immunol. 1972; 109(5):1017-21. View

Carrel S, BARANDUN S . Protein-containing polyacrylamide gels: their use as immunoadsorbents of high capacity. Immunochemistry. 1971; 8(1):39-48. DOI: 10.1016/0019-2791(71)90420-4. View

Neurath A, Strick N . Host specificity of a serum marker for hepatitis B: evidence that "e antigen" has the properties of an immunoglobulin. Proc Natl Acad Sci U S A. 1977; 74(4):1702-6. PMC: 430861. DOI: 10.1073/pnas.74.4.1702. View

Fields H, Bradley D, Davis C, MAYNARD J . Purification and partial characterization of hepatitis e antigen (HBeAg). Infect Immun. 1978; 20(3):792-803. PMC: 421928. DOI: 10.1128/iai.20.3.792-803.1978. View

Alter H, Seeff L, KAPLAN P, McAuliffe V, Wright E, Gerin J . Type B hepatitis: the infectivity of blood positive for e antigen and DNA polymerase after accidental needlestick exposure. N Engl J Med. 1976; 295(17):909-13. DOI: 10.1056/NEJM197610212951701. View

Hunter W, GREENWOOD F . Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962; 194:495-6. DOI: 10.1038/194495a0. View

Frosner G, Brodersen M, Papaevangelou G, Sugg U, Haas H, MUSHAHWAR I . Detection of HBeAg and anti-HBe in acute hepatitis B by a sensitive radioimmunoassay. J Med Virol. 1978; 3(1):67-76. DOI: 10.1002/jmv.1890030114. View

10.

Dolken G, Klein G . A solid-phase radioimmunoassay for Epstein-Barr virus-associated membrane antigen prepared from B95-8 cell culture supernatants. J Natl Cancer Inst. 1977; 58(5):1239-45. DOI: 10.1093/jnci/58.5.1239. View

11.

Nielsen J, Dietrichson O, Juhl E . Incidence and meaning of the "e" determinant among hepatitis-B-antigen positive patients with acute and chronic liver diseases. Report from the Copenhagen Hepatitis Acuta Programme. Lancet. 1974; 2(7886):913-5. DOI: 10.1016/s0140-6736(74)91127-1. View

12.

Gorsky Y, Sulitzeanu D . A radioactive antibody binding-inhibition assay, for the detection of cell-membrane related antigens in body fluids. J Immunol Methods. 1975; 6(3):291-300. DOI: 10.1016/0022-1759(75)90072-1. View

13.

VYAS G, Peterson D, Townsend R, Damle S, Magnius L . Hepatitis B "e" antigen: an apparent association with lactate dehydrogenase isozyme-5. Science. 1977; 198(4321):1068-70. DOI: 10.1126/science.73221. View

14.

Nordenfelt E, Kjellen L . Dane particles, DNA polymerase, and e-antigen in two different categories of hepatitis B antigen carriers. Intervirology. 1975; 5(3-4):225-32. DOI: 10.1159/000149918. View

15.

Dolken G, Klein G . Radioimmunoassay for Epstein--Barr virus (EBV)-associated nuclear antigen (EBNA). Binding of iodinated antibodies to antigen immobilized in polyacrylamide gel. Eur J Cancer (1965). 1977; 13(11):1277-86. DOI: 10.1016/0014-2964(77)90036-6. View

16.

Okada K, Kamiyama I, Inomata M, Imai M, Miyakawa Y . e antigen and anti-e in the serum of asymptomatic carrier mothers as indicators of positive and negative transmission of hepatitis B virus to their infants. N Engl J Med. 1976; 294(14):746-9. DOI: 10.1056/NEJM197604012941402. View

17.

Nordenfelt E . Dane particle-associated DNA polymerase and e antigen: relation to chronic hepatitis among carriers of hepatitis B surface antigen. J Infect Dis. 1976; 134(1):85-9. DOI: 10.1093/infdis/134.1.85. View