Glycosaminoglycans in Crystal-surface Binding Substances and Their Role in Calcium Oxalate Crystal Growth
Affiliations
Objective: To clarify the role of glycosaminoglycans (GAGs) in crystal-surface binding substances (CSBS) on the growth of calcium oxalate crystals in urine.
Materials And Methods: Urine samples (24 h) were collected from healthy men (aged 25-42 years) and CSBS were obtained from the pooled urine samples. The CSBS were digested with heparitinase or proteinase and the inhibition of crystal growth assessed before and after enzyme digestion. Anion-exchange chromatography and high-performance liquid chromatography (HPLC) were used to determine the types of GAGs contained in the CSBS.
Results: The inhibitory activity of CSBS on crystal growth decreased with concentration when digested with heparitinase or proteinase. HPLC showed that CSBS contained a small amount of dermatan sulphate and abundant heparan sulphate, both of which inhibited crystal growth.
Conclusion: Both heparan sulphate and dermatan sulphate may inhibit calcium oxalate crystallization, the former being the predominant GAG in CSBS.
study on heparin/poly-l-lysine-copper coating for surface functionalization of ureteral stent.
Awonusi B, Li J, Li H, Wang Z, Hu J, Yang K Regen Biomater. 2022; 9:rbac083.
PMID: 36338173 PMC: 9629973. DOI: 10.1093/rb/rbac083.
Awonusi B, Li J, Li H, Wang Z, Yang K, Zhao J Regen Biomater. 2022; 9:rbac047.
PMID: 35928999 PMC: 9345062. DOI: 10.1093/rb/rbac047.
Update on ureteral stent technology.
Lange D, Chew B Ther Adv Urol. 2011; 1(3):143-8.
PMID: 21789062 PMC: 3126057. DOI: 10.1177/1756287209341306.