Direct Action of Nitric Oxide on Osteoblastic Differentiation

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 1997 Jun 30

PMID 9237637

Citations 19

Authors

H Hikiji

W S Shin

S Oida

T Takato

T Koizumi

T Toyo-oka

Affiliations

Soon will be listed here.

Abstract

The effect of nitric oxide (NO) on osteoblastic differentiation was examined in cultured mouse osteoblasts. Interleukin-1beta and tumor necrosis factor-alpha expressed inducible NO synthase gene with little effect on constitutive NO synthase gene. These cytokines increased NO production, which was inhibited by L-NMMA pretreatment, and decreased alkaline phosphatase (AIPase) activity, which was not restored by L-NMMA. Furthermore, NO donors, sodium nitroprusside and NONOate dose-dependently elevated AIPase activity and expression of osteocalcin gene. These results suggest that NO directly facilitates osteoblastic differentiation and the cytokine-induced inhibition of AIPase activity is mediated via mechanism other than NO.

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