A New Combination of Protecting Groups and Links for Encoded Synthetic Libraries Suited for Consecutive Tests on the Solid Phase and in Solution

Overview

Journal Mol Divers

Date 1996 Feb 1

PMID 9237199

Citations 1

Authors

E R Felder

G Heizmann

I T Matthews

H RINK

E Spieser

Affiliations

Soon will be listed here.

Abstract

A strategy for high-throughput evaluation of combinatorial compound libraries is reported, which circumvents the necessity to test complex mixtures. The method is based on a new combination of protecting groups, solid-phase linker and tags. The bulk of the library first undergoes a binding assay with the components grafted on beads. A selection of beads carrying strong ligands is stripped from the labelled target and distributed into microvessels. The ligands are cleaved and rinsed into microeluates. Subsequently, a more detailed characterization with a functional assay in solution determines the best performers, which are identified through the peptidic tag left behind on the corresponding mother bead.

Citing Articles

Peptomers: a versatile approach for the preparation of diverse combinatorial peptidomimetic bead libraries.

Ostergaard S, Holm A Mol Divers. 1997; 3(1):17-27.

PMID: 9527474 DOI: 10.1023/a:1009698507588.

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