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Optimization of the Performance of the Polymerase Chain Reaction in Silicon-based Microstructures

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Specialty Biochemistry
Date 1997 Aug 1
PMID 9224619
Citations 18
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Abstract

We have demonstrated the ability to perform real-time homogeneous, sequence specific detection of PCR products in silicon microstructures. Optimal design/ processing result in equivalent performance (yield and specificity) for high surface-to-volume silicon structures as compared to larger volume reactions in polypropylene tubes. Amplifications in volumes as small as 0.5 microl and thermal cycling times reduced as much as 5-fold from that of conventional systems have been demonstrated for the microstructures.

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