Detection of Human Papillomavirus Infection in Squamous Tumours of the Conjunctiva and Lacrimal Sac by Immunohistochemistry, in Situ Hybridisation, and Polymerase Chain Reaction

Overview

Journal Br J Ophthalmol

Specialty Ophthalmology

Date 1997 Apr 1

PMID 9215061

Citations 31

Authors

Y Nakamura

Y Mashima

K Kameyama

M Mukai

Y Oguchi

Affiliations

Soon will be listed here.

Abstract

Background: Squamous tumours of the ocular surface, including the lacrimal pathway, range from benign lesions to invasive carcinomas. Some of these tumours are associated with human papillomavirus (HPV) infection, with the types of HPV differing among papillomas and dysplastic or malignant lesions.

Methods: The relation between squamous tumours of the conjunctiva and lacrimal sac and HPV infection was investigated in 17 individuals with such tumours. Nine of the 17 tumours were benign, four were dysplastic lesions, and four were carcinomas.

Results: Eight specimens showed positive immunohistochemical staining with antibodies to HPV; four of these eight were papillomas, three were dysplastic lesions, and one was a carcinoma. Koilocytosis was detected in seven of these eight tumours. Five of the eight specimens positive for immunohistochemical staining were also positive for HPV DNA by in situ hybridisation, and all eight were positive for HPV DNA by the polymerase chain reaction (PCR) method.

Conclusion: Approximately 50% of squamous tumours of the ocular surface and lacrimal sac were associated with HPV infection. This is the first report, to our knowledge, of the detection of HPV in the field of ophthalmology by a combination of immunohistochemistry, in situ hybridisation, and PCR.

Citing Articles

Esophageal squamous papilloma: Literature review and case-control retrospective study with histopathological exam of human papillomavirus.

Ahmad A, Lee A, Nithagon P, Ayaz O, Altork N, Peppas S JGH Open. 2023; 7(10):674-681.

PMID: 37908288 PMC: 10615170. DOI: 10.1002/jgh3.12942.

Human papillomavirus-related neoplasia of the ocular adnexa.

Ramberg I Acta Ophthalmol. 2022; 100 Suppl 272:3-33.

PMID: 36203222 PMC: 9827891. DOI: 10.1111/aos.15244.

Detection of Human Papillomavirus in Squamous Lesions of the Conjunctiva Using RNA and DNA In-Situ Hybridization.

Peterson C, Parikh R, Ahmad M, Campbell A, Daoud Y, Mahoney N Int J Mol Sci. 2022; 23(13).

PMID: 35806252 PMC: 9266440. DOI: 10.3390/ijms23137249.

Epstein-Barr Virus, But Not Human Papillomavirus, Is Associated With Preinvasive and Invasive Ocular Surface Squamous Neoplasias in Zambian Patients.

Julius P, Siyumbwa S, Moonga P, Maate F, Kaile T, Haynatski G Front Oncol. 2022; 12:864066.

PMID: 35494029 PMC: 9047892. DOI: 10.3389/fonc.2022.864066.

Human Papillomavirus Related Neoplasia of the Ocular Adnexa.

Ramberg I, Heegaard S Viruses. 2021; 13(8).

PMID: 34452388 PMC: 8402806. DOI: 10.3390/v13081522.

References

Fujinaga Y, Shimada M, Okazawa K, Fukushima M, Kato I, Fujinaga K . Simultaneous detection and typing of genital human papillomavirus DNA using the polymerase chain reaction. J Gen Virol. 1991; 72 ( Pt 5):1039-44. DOI: 10.1099/0022-1317-72-5-1039. View

McDonnell J, McDonnell P, Sun Y . Human papillomavirus DNA in tissues and ocular surface swabs of patients with conjunctival epithelial neoplasia. Invest Ophthalmol Vis Sci. 1992; 33(1):184-9. View

Dekmezian R, Chen X, Kuo T, Ordonez N, Katz R . DNA hybridization for human papillomavirus (HPV) in cervical lesions. Relationship of the presence of various viral subtypes to expression of HPV structural proteins, involucrin, and carcinoembryonic antigen. Arch Pathol Lab Med. 1987; 111(1):22-7. View

Tsutsumi K, Nakajima T, Gotoh M, Shimosato Y, Tsunokawa Y, Terada M . In situ hybridization and immunohistochemical study of human papillomavirus infection in adult laryngeal papillomas. Laryngoscope. 1989; 99(1):80-5. DOI: 10.1288/00005537-198901000-00015. View

McDonnell J, Mayr A, Martin W . DNA of human papillomavirus type 16 in dysplastic and malignant lesions of the conjunctiva and cornea. N Engl J Med. 1989; 320(22):1442-6. DOI: 10.1056/NEJM198906013202202. View

Naghashfar Z, McDonnell P, McDonnell J, Green W, Shah K . Genital tract papillomavirus type 6 in recurrent conjunctival papilloma. Arch Ophthalmol. 1986; 104(12):1814-5. DOI: 10.1001/archopht.1986.01050240088046. View

Hsu S, RAINE L, FANGER H . Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981; 29(4):577-80. DOI: 10.1177/29.4.6166661. View

Dover J, Arndt K . Dermatology. JAMA. 1992; 268(3):342-4. View

Odrich M, Jakobiec F, Lancaster W, Kenyon K, Kelly L, Kornmehl E . A spectrum of bilateral squamous conjunctival tumors associated with human papillomavirus type 16. Ophthalmology. 1991; 98(5):628-35. DOI: 10.1016/s0161-6420(91)32218-8. View

10.

Lass J, Jenson A, Papale J, Albert D . Papillomavirus in human conjunctival papillomas. Am J Ophthalmol. 1983; 95(3):364-8. DOI: 10.1016/s0002-9394(14)78307-2. View

11.

Hoshikawa T, Nakajima T, Uhara H, Gotoh M, Shimosato Y, Tsutsumi K . Detection of human papillomavirus DNA in laryngeal squamous cell carcinomas by polymerase chain reaction. Laryngoscope. 1990; 100(6):647-50. DOI: 10.1288/00005537-199006000-00018. View

12.

Goelz S, Hamilton S, Vogelstein B . Purification of DNA from formaldehyde fixed and paraffin embedded human tissue. Biochem Biophys Res Commun. 1985; 130(1):118-26. DOI: 10.1016/0006-291x(85)90390-0. View

13.

Ashinoff R, Li J, Jacobson M, Friedman-Kien A, Geronemus R . Detection of human papillomavirus DNA in squamous cell carcinoma of the nail bed and finger determined by polymerase chain reaction. Arch Dermatol. 1991; 127(12):1813-8. View

14.

Gupta J, Pilotti S, Shah K, De Palo G, RILKE F . Human papillomavirus-associated early vulvar neoplasia investigated by in situ hybridization. Am J Surg Pathol. 1987; 11(6):430-4. DOI: 10.1097/00000478-198706000-00003. View

15.

Crum C, Ikenberg H, Richart R, Gissman L . Human papillomavirus type 16 and early cervical neoplasia. N Engl J Med. 1984; 310(14):880-3. DOI: 10.1056/NEJM198404053101403. View

16.

McDonnell P, McDonnell J, Kessis T, Green W, Shah K . Detection of human papillomavirus type 6/11 DNA in conjunctival papillomas by in situ hybridization with radioactive probes. Hum Pathol. 1987; 18(11):1115-9. DOI: 10.1016/s0046-8177(87)80378-7. View

17.

Durst M, Gissmann L, Ikenberg H, zur Hausen H . A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proc Natl Acad Sci U S A. 1983; 80(12):3812-5. PMC: 394142. DOI: 10.1073/pnas.80.12.3812. View

18.

de Villiers E, Weidauer H, Otto H, zur Hausen H . Papillomavirus DNA in human tongue carcinomas. Int J Cancer. 1985; 36(5):575-8. DOI: 10.1002/ijc.2910360510. View

19.

Multhaupt H, Fessler J, Warhol M . Detection of human papillomavirus in laryngeal lesions by in situ hybridization. Hum Pathol. 1994; 25(12):1302-5. DOI: 10.1016/0046-8177(94)90089-2. View

20.

McCance D, Campion M, Clarkson P, Chesters P, Jenkins D, Singer A . Prevalence of human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and invasive carcinoma of the cervix. Br J Obstet Gynaecol. 1985; 92(11):1101-5. DOI: 10.1111/j.1471-0528.1985.tb03019.x. View