The LIM-only Protein Lmo2 is a Bridging Molecule Assembling an Erythroid, DNA-binding Complex Which Includes the TAL1, E47, GATA-1 and Ldb1/NLI Proteins

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1997 Jun 2

PMID 9214632

Citations 354

Authors

I A Wadman

H Osada

G G Grutz

A D Agulnick

H Westphal

A Forster

T H Rabbitts

Affiliations

Soon will be listed here.

Abstract

The LIM-only protein Lmo2, activated by chromosomal translocations in T-cell leukaemias, is normally expressed in haematopoiesis. It interacts with TAL1 and GATA-1 proteins, but the function of the interaction is unexplained. We now show that in erythroid cells Lmo2 forms a novel DNA-binding complex, with GATA-1, TAL1 and E2A, and the recently identified LIM-binding protein Ldb1/NLI. This oligomeric complex binds to a unique, bipartite DNA motif comprising an E-box, CAGGTG, followed approximately 9 bp downstream by a GATA site. In vivo assembly of the DNA-binding complex requires interaction of all five proteins and establishes a transcriptional transactivating complex. These data demonstrate one function for the LIM-binding protein Ldb1 and establish a function for the LIM-only protein Lmo2 as an obligatory component of an oligomeric, DNA-binding complex which may play a role in haematopoiesis.

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