Growth of Silicone-immobilized Bacteria on Polycarbonate Membrane Filters, a Technique to Study Microcolony Formation Under Anaerobic Conditions

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1997 Jul 1

PMID 9212439

Citations 12

Authors

O Hojberg

S J Binnerup

J Sorensen

Affiliations

Soon will be listed here.

Abstract

A technique was developed to study microcolony formation by silicone-immobilized bacteria on polycarbonate membrane filters under anaerobic conditions. A sudden shift to anaerobiosis was obtained by submerging the filters in medium which was depleted for oxygen by a pure culture of bacteria. The technique was used to demonstrate that preinduction of nitrate reductase under low-oxygen conditions was necessary for nonfermenting, nitrate-respiring bacteria, e.g., Pseudomonas spp., to cope with a sudden lack of oxygen. In contrast, nitrate-respiring, fermenting bacteria, e.g., Bacillus and Escherichia spp., formed microcolonies under anaerobic conditions with or without the presence of nitrate and irrespective of aerobic or anaerobic preculture conditions.

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References

Jannasch H . Studies on planktonic bacteria by means of a direct membrane filter method. J Gen Microbiol. 1958; 18(3):609-20. DOI: 10.1099/00221287-18-3-609. View

Poulsen L, Ballard G, Stahl D . Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl Environ Microbiol. 1993; 59(5):1354-60. PMC: 182089. DOI: 10.1128/aem.59.5.1354-1360.1993. View

Knowles R . Denitrification. Microbiol Rev. 1982; 46(1):43-70. PMC: 373209. DOI: 10.1128/mr.46.1.43-70.1982. View

Winding A, Binnerup S, Sorensen J . Viability of indigenous soil bacteria assayed by respiratory activity and growth. Appl Environ Microbiol. 1994; 60(8):2869-75. PMC: 201736. DOI: 10.1128/aem.60.8.2869-2875.1994. View

Zumft W, Vega J . Reduction of nitrite to nitrous oxide by a cytoplasmic membrane fraction from the marine denitrifier Pseudomonas perfectomarinus. Biochim Biophys Acta. 1979; 548(3):484-99. DOI: 10.1016/0005-2728(79)90060-4. View

Rodrigues U, Kroll R . Rapid detection of salmonellas in raw meats using a fluorescent antibody-microcolony technique. J Appl Bacteriol. 1990; 68(3):213-23. DOI: 10.1111/j.1365-2672.1990.tb02567.x. View

Rodrigues U, Kroll R . Microcolony epifluorescence microscopy for selective enumeration of injured bacteria in frozen and heat-treated foods. Appl Environ Microbiol. 1989; 55(4):778-87. PMC: 184202. DOI: 10.1128/aem.55.4.778-787.1989. View

Payne W, RILEY P, Cox Jr C . Separate nitrite, nitric oxide, and nitrous oxide reducing fractions from Pseudomonas perfectomarinus. J Bacteriol. 1971; 106(2):356-61. PMC: 285104. DOI: 10.1128/jb.106.2.356-361.1971. View

de Bruyn J, Boogerd F, Bos P, Kuenen J . Floating filters, a novel technique for isolation and enumeration of fastidious, acidophilic, iron-oxidizing, autotrophic bacteria. Appl Environ Microbiol. 1990; 56(9):2891-4. PMC: 184860. DOI: 10.1128/aem.56.9.2891-2894.1990. View

10.

Seidler E . The tetrazolium-formazan system: design and histochemistry. Prog Histochem Cytochem. 1991; 24(1):1-86. DOI: 10.1016/s0079-6336(11)80060-4. View

11.

Prosser J . Molecular marker systems for detection of genetically engineered micro-organisms in the environment. Microbiology (Reading). 1994; 140 ( Pt 1):5-17. DOI: 10.1099/13500872-140-1-5. View

12.

Aida T, Hata S, Kusunoki H . Temporary low oxygen conditions for the formation of nitrate reductase and nitrous oxide reductase by denitrifying Pseudomonas sp. G59. Can J Microbiol. 1986; 32(7):543-7. DOI: 10.1139/m86-101. View

13.

Korner H, Zumft W . Expression of denitrification enzymes in response to the dissolved oxygen level and respiratory substrate in continuous culture of Pseudomonas stutzeri. Appl Environ Microbiol. 1989; 55(7):1670-6. PMC: 202933. DOI: 10.1128/aem.55.7.1670-1676.1989. View

14.

Binnerup S, Sorensen J . Nitrate and nitrite microgradients in barley rhizosphere as detected by a highly sensitive denitrification bioassay. Appl Environ Microbiol. 1992; 58(8):2375-80. PMC: 195789. DOI: 10.1128/aem.58.8.2375-2380.1992. View

15.

POSTGATE J, CRUMPTON J, Hunter J . The measurement of bacterial viabilities by slide culture. J Gen Microbiol. 1961; 24:15-24. DOI: 10.1099/00221287-24-1-15. View

16.

Winter F, York G, El-Nakhal H . Quick counting method for estimating the number of viable microbes on food and food processing equipment. Appl Microbiol. 1971; 22(1):89-92. PMC: 377381. DOI: 10.1128/am.22.1.89-92.1971. View

17.

Rodrigues U, Kroll R . Rapid selective enumeration of bacteria in foods using a microcolony epifluorescence microscopy technique. J Appl Bacteriol. 1988; 64(1):65-78. DOI: 10.1111/j.1365-2672.1988.tb02430.x. View