Identification of a Minimal Promoter Element of the Mouse Epidermal Growth Factor Gene

Overview

Journal Biochem J

Specialty Biochemistry

Date 1997 Jun 15

PMID 9210411

Citations 2

Authors

J C Pascall

K D Brown

Affiliations

Soon will be listed here.

Abstract

We have previously generated a transgenic mouse line (EGF/Tag) in which simian virus 40 (SV40) T-antigen expression is directed by the mouse epidermal growth factor (EGF) gene promoter. In these mice, cellular hyperproliferation is observed in the submaxillary gland associated with SV40 T-antigen expression. In addition, SV40 T-antigen-expressing tumours of prostatic origin are seen. We have now derived immortalized cell lines from these tissues and have used the cells to perform a functional analysis of the EGF gene promoter. Cells were transfected with EGF promoter/reporter constructs, and an element located between 51 and 35 bases upstream of the EGF mRNA start site required for basal activity of the promoter was identified. Electrophoretic mobility-shift analysis suggests that three proteins bind to this region, one of which is either Sp1 or a closely related protein.

Citing Articles

Role of post-translational modifications of Sp1 in cancer: state of the art.

Sun X, Xiao C, Wang X, Wu S, Yang Z, Sui B Front Cell Dev Biol. 2024; 12:1412461.

PMID: 39228402 PMC: 11368732. DOI: 10.3389/fcell.2024.1412461.

Beyond HAT Adaptor: TRRAP Liaisons with Sp1-Mediated Transcription.

Yin B, Wang Z Int J Mol Sci. 2021; 22(22).

PMID: 34830324 PMC: 8625110. DOI: 10.3390/ijms222212445.

References

Sartorelli V, Webster K, Kedes L . Muscle-specific expression of the cardiac alpha-actin gene requires MyoD1, CArG-box binding factor, and Sp1. Genes Dev. 1990; 4(10):1811-22. DOI: 10.1101/gad.4.10.1811. View

Cohen S . Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem. 1962; 237:1555-62. View

Chen X, Azizkhan J, Lee D . The binding of transcription factor Sp1 to multiple sites is required for maximal expression from the rat transforming growth factor alpha promoter. Oncogene. 1992; 7(9):1805-15. View

Rall L, Scott J, Bell G, Crawford R, Penschow J, Niall H . Mouse prepro-epidermal growth factor synthesis by the kidney and other tissues. Nature. 1985; 313(5999):228-31. DOI: 10.1038/313228a0. View

Pascall J, Brown K . Structural analysis of the 5'-flanking sequence of the mouse epidermal growth factor gene. J Mol Endocrinol. 1988; 1(1):5-11. DOI: 10.1677/jme.0.0010005. View

Carpenter G, Cohen S . Epidermal growth factor. J Biol Chem. 1990; 265(14):7709-12. View

Scott J, Urdea M, Quiroga M, Fong N, Selby M, Rutter W . Structure of a mouse submaxillary messenger RNA encoding epidermal growth factor and seven related proteins. Science. 1983; 221(4607):236-40. DOI: 10.1126/science.6602382. View

Hagen G, Muller S, Beato M, Suske G . Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes. Nucleic Acids Res. 1992; 20(21):5519-25. PMC: 334381. DOI: 10.1093/nar/20.21.5519. View

Lemaigre F, Courtois S, Lafontaine D, Rousseau G . Evidence that the upstream stimulatory factor and the Sp1 transcription factor bind in vitro to the promoter of the human-growth-hormone gene. Eur J Biochem. 1989; 181(3):555-61. DOI: 10.1111/j.1432-1033.1989.tb14760.x. View

10.

Sigmund C, Okuyama K, Ingelfinger J, Jones C, Mullins J, Kane C . Isolation and characterization of renin-expressing cell lines from transgenic mice containing a renin-promoter viral oncogene fusion construct. J Biol Chem. 1990; 265(32):19916-22. View

11.

Dennig J, Hagen G, Beato M, Suske G . Members of the Sp transcription factor family control transcription from the uteroglobin promoter. J Biol Chem. 1995; 270(21):12737-44. DOI: 10.1074/jbc.270.21.12737. View

12.

Lemaire P, Vesque C, Schmitt J, Stunnenberg H, Frank R, Charnay P . The serum-inducible mouse gene Krox-24 encodes a sequence-specific transcriptional activator. Mol Cell Biol. 1990; 10(7):3456-67. PMC: 360781. DOI: 10.1128/mcb.10.7.3456-3467.1990. View

13.

Gruda M, Zabolotny J, Xiao J, Davidson I, Alwine J . Transcriptional activation by simian virus 40 large T antigen: interactions with multiple components of the transcription complex. Mol Cell Biol. 1993; 13(2):961-9. PMC: 358980. DOI: 10.1128/mcb.13.2.961-969.1993. View

14.

Pascall I, Surani M, Barton S, Vaughan T, Brown K . Directed expression of simian virus 40 T-antigen in transgenic mice using the epidermal growth factor gene promoter. J Mol Endocrinol. 1994; 12(3):313-25. DOI: 10.1677/jme.0.0120313. View

15.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

16.

Kurachi H, Oka T . Regulation of the level of epidermal growth factor by oestrogen in the submandibular gland of female mice. J Endocrinol. 1986; 109(2):221-5. DOI: 10.1677/joe.0.1090221. View

17.

Pascall J, Saunders J, Blakeley D, Laurie M, Brown K . Tissue-specific effects of castration and ovariectomy on murine epidermal growth factor and its mRNA. J Endocrinol. 1989; 121(3):501-6. DOI: 10.1677/joe.0.1210501. View

18.

Corps A, Pascall J, Hadfield K, Brown K . Identification of a functional promoter element in the 5'-flanking region of the rat cMG1/TIS11b gene. Biochem J. 1995; 311 ( Pt 1):251-8. PMC: 1136146. DOI: 10.1042/bj3110251. View

19.

Windle J, Weiner R, Mellon P . Cell lines of the pituitary gonadotrope lineage derived by targeted oncogenesis in transgenic mice. Mol Endocrinol. 1990; 4(4):597-603. DOI: 10.1210/mend-4-4-597. View

20.

Chen X, Wright K, Berkowitz E, Azizkhan J, Ting J, Lee D . Protein interactions at Sp1-like sites in the TGF alpha promoter as visualized by in vivo genomic footprinting. Oncogene. 1994; 9(11):3179-87. View