Three-dimensional Culture of Bovine Chondrocytes in Rotating-wall Vessels

Overview

Journal In Vitro Cell Dev Biol Anim

Publisher Springer

Specialties Biology
Cell Biology

Date 1997 May 1

PMID 9196894

Citations 13

Authors

T L Baker

T J Goodwin

Affiliations

Soon will be listed here.

Abstract

The Rotating-Wall Vessel (RWV) was used to culture chondrocytes for 36 d to observe the influence of low-shear and quiescent culture conditions allowing three-dimensional freedom on growth, differentiation, and extracellular matrix formation. Chondrocytes were freshly isolated from bovine cartilage and placed into the RWV with Cytodex-3 microcarriers. Nonadherent petri dishes were initiated with microcarriers as representative of standard culture conditions. In the RWV, large three-dimensional aggregates (5-7 mm) were formed in suspension. In addition, a large sheet of matrix adhered to the oxygenator core and vessel endcaps. Petri dish culture resulted in the formation of sheets of chondrocytes with no matrix production. Immunocytochemical analyses on histologic sections of tissue obtained from the RWV and the petri dish controls were performed with antibodies against fibronectin, collagen II, chondroitin-4-sulfate, chondroitin-6-sulfate, and vimentin. Results demonstrated increased signal in the RWV material while the petri dishes demonstrated a slight decrease in signal. In addition, differentiated chondrocytes were observed in sections of RWV material through 36 d, while few were observed in the sections of petri dish material. These results indicate that the unique conditions provided by the RWV afford access to cellular processes that signify the initiation of differentiation as well as production of normal matrix material.

Citing Articles

Structural and Molecular Changes of Human Chondrocytes Exposed to the Rotating Wall Vessel Bioreactor.

Steinwerth P, Bertrand J, Sandt V, Marchal S, Sahana J, Bollmann M Biomolecules. 2024; 14(1).

PMID: 38254625 PMC: 10813504. DOI: 10.3390/biom14010025.

Formation of three-dimensional cell aggregates expressing lens-specific proteins in various cultures of human iris-derived tissue cells and iPS cells.

Hiramatsu N, Yamamoto N, Kato Y, Nagai N, Isogai S, Imaizumi K Exp Ther Med. 2022; 24(2):539.

PMID: 35837031 PMC: 9257972. DOI: 10.3892/etm.2022.11476.

The Application of Bioreactors for Cartilage Tissue Engineering: Advances, Limitations, and Future Perspectives.

Fu L, Li P, Li H, Gao C, Yang Z, Zhao T Stem Cells Int. 2021; 2021:6621806.

PMID: 33542736 PMC: 7843191. DOI: 10.1155/2021/6621806.

LRP receptors in chondrocytes are modulated by simulated microgravity and cyclic hydrostatic pressure.

Nordberg R, Mellor L, Krause A, Donahue H, Loboa E PLoS One. 2019; 14(10):e0223245.

PMID: 31584963 PMC: 6777824. DOI: 10.1371/journal.pone.0223245.

Development of a whole organ culture model for intervertebral disc disease.

Stannard J, Edamura K, Stoker A, OConnell G, Kuroki K, Hung C J Orthop Translat. 2018; 5:1-8.

PMID: 30035069 PMC: 5987001. DOI: 10.1016/j.jot.2015.08.002.

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