Negative Regulation in the Expression of a Sugar-inducible Gene in Arabidopsis Thaliana. A Recessive Mutation Causing Enhanced Expression of a Gene for Beta-amylase
Overview
Authors
Affiliations
Expression of a beta-amylase gene of Arabidopsis thaliana (AT beta-Amy) is regulated by sugars. We identified a mutant, hba1, in which the level of expression of AT beta-Amy in leaves of plants that had been grown in a medium with 2% sucrose was significantly higher than that in wild-type plants. Higher that wild-type levels of beta-amylase in hba1 plants depended on the presence of 1 to 2% sucrose or 1% glucose in the medium, whereas leaves of mutant plants grown with higher levels of sugars had beta-amylase activities similar to those in leaves of wild-type plants. The hba1 phenotype was recessive and did not affect levels of sugars and starch in leaves. It is proposed that expression of AT beta-Amy is regulated by a combination of both positive and negative factors, dependent on the level of sugars, and that HBA1 might function to maintain low-level expression of AT beta-Amy until the level of sugars reaches some high level. Results of crosses of hba1 plants with transgenic plants that harbored an AT beta-Amy:GUS transgene with 1587 bp of the 5'-upstream region suggested that HBA1 affects expressions of AT beta-Amy in trans. The hba1 plants also had growth defects and elevated levels of anthocyanin in their petioles. However, sugar-related changes in levels of several mRNAs other than beta-amylase mRNA were unaffected in hba1 plants, suggesting that only a subset of sugar-regulated genes is under the control HBA1.
Tan Y, Wen B, Xu L, Zong X, Sun Y, Wei G Front Plant Sci. 2023; 14:1079292.
PMID: 36860903 PMC: 9968857. DOI: 10.3389/fpls.2023.1079292.
Glucose Supply Induces PsMYB2-Mediated Anthocyanin Accumulation in 'Tai Yang' Cut Flower.
Zhang L, Yan L, Zhang C, Kong X, Zheng Y, Dong L Front Plant Sci. 2022; 13:874526.
PMID: 35774824 PMC: 9237572. DOI: 10.3389/fpls.2022.874526.
Zou B, Wan D, Li R, Han X, Li G, Wang R PLoS One. 2017; 12(3):e0173129.
PMID: 28253311 PMC: 5333885. DOI: 10.1371/journal.pone.0173129.
Chen L, Huang Y, Xu M, Cheng Z, Zhang D, Zheng J PLoS One. 2016; 11(7):e0159238.
PMID: 27415428 PMC: 4944901. DOI: 10.1371/journal.pone.0159238.
Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis.
Heisel T, Li C, Grey K, Gibson S Front Plant Sci. 2013; 4:245.
PMID: 23882272 PMC: 3713338. DOI: 10.3389/fpls.2013.00245.