Reserpine-induced Immunocytochemical Change of Neuropeptide Y in the Hypothalamic Arcuate Nucleus

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1996 Feb 1

PMID 9182248

Authors

H Okamura

T Sugano

Y Ibata

Affiliations

Soon will be listed here.

Abstract

The effect of reserpine on neuropeptide Y immunoreactive (NPY-IR) neurons in the rat hypothalamic arcuate nucleus was examined by immunocytochemical techniques. Although only NPY-IR fibers and terminals were distributed in this nucleus in untreated and saline treated rats, single treatment of reserpine (10 mg/kg, i.p.) visualized abundant NPY-IR neuronal cell bodies: the increase began at 12 h of postinjection, reached its maximal level at 48 h, and returned to its normal level at 96 h. Pretreatment of nialamide, a monoamine oxidase inhibitor, prevented these acute reserpine-induced changes, suggesting reserpine acts on NPY neurons through monoaminergic mechanism. Chronic treatment of haloperidol (5 mg/kg, once daily for 5 days) a dopamine receptor antagonist, could induce the similar increase of NPY immunoreactivity. However, interruption of adrenergic and serotonergic neurotransmissions by chronic treatment of propranorol and methysergide, or chemical lesions of ascending noradrenergic and serotonergic pathways by 6-hydroxydopamine and 5,6-dihydroxytryptamine, could not induce any immunoreactive increase of NPY in arcuate neurons. These findings strongly suggest that reserpine-induced NPY increase occurs through dopaminergic afferents in hypothalamic arcuate neurons.

References

Schwartz M, Sipols A, MARKS J, Sanacora G, White J, Scheurink A . Inhibition of hypothalamic neuropeptide Y gene expression by insulin. Endocrinology. 1992; 130(6):3608-16. DOI: 10.1210/endo.130.6.1597158. View

Clark J, Kalra P, Crowley W, Kalra S . Neuropeptide Y and human pancreatic polypeptide stimulate feeding behavior in rats. Endocrinology. 1984; 115(1):427-9. DOI: 10.1210/endo-115-1-427. View

Miyake M, Iguchi K, Okamura H, Fukui K, Nakajima T, Chihara K . Effect of haloperidol on immunoreactive neuropeptide Y in rat cerebral cortex and basal ganglia. Brain Res Bull. 1990; 25(2):263-9. DOI: 10.1016/0361-9230(90)90071-7. View

Chua Jr S, Leibel R, Hirsch J . Food deprivation and age modulate neuropeptide gene expression in the murine hypothalamus and adrenal gland. Brain Res Mol Brain Res. 1991; 9(1-2):95-101. DOI: 10.1016/0169-328x(91)90134-j. View

Smialowska M, Legutko B . Haloperidol-induced increase in neuropeptide Y immunoreactivity in the locus coeruleus of the rat brain. Neuroscience. 1992; 47(2):351-5. DOI: 10.1016/0306-4522(92)90251-v. View

Bai F, Yamano M, Shiotani Y, Emson P, Smith A, Powell J . An arcuato-paraventricular and -dorsomedial hypothalamic neuropeptide Y-containing system which lacks noradrenaline in the rat. Brain Res. 1985; 331(1):172-5. DOI: 10.1016/0006-8993(85)90730-9. View

Lundberg J, Saria A, Franco-Cereceda A, Hokfelt T, Terenius L, Goldstein M . Differential effects of reserpine and 6-hydroxydopamine on neuropeptide Y (NPY) and noradrenaline in peripheral neurons. Naunyn Schmiedebergs Arch Pharmacol. 1985; 328(3):331-40. DOI: 10.1007/BF00515563. View

Bernardis L, Bellinger L . The dorsomedial hypothalamic nucleus revisited: 1986 update. Brain Res. 1987; 434(3):321-81. DOI: 10.1016/0165-0173(87)90004-x. View

Lundberg J, Saria A, Franco-Cereceda A . Mechanisms underlying changes in the contents of neuropeptide Y in cardiovascular nerves and adrenal gland induced by sympatholytic drugs. Acta Physiol Scand. 1985; 124(4):603-11. DOI: 10.1111/j.1748-1716.1985.tb00054.x. View

10.

Smialowska M, Legutko B . Influence of reserpine administration on neuropeptide Y immunoreactivity in the locus coeruleus and caudate-putamen nucleus of the rat brain. Neuroscience. 1990; 36(2):411-5. DOI: 10.1016/0306-4522(90)90436-8. View

11.

Lundberg J, Saria A . Reserpine-induced depletion of neuropeptide Y from cardiovascular nerves and adrenal gland due to enhanced release. Naunyn Schmiedebergs Arch Pharmacol. 1986; 332(2):163-8. DOI: 10.1007/BF00511407. View

12.

Sawchenko P, Swanson L, Grzanna R, Howe P, Bloom S, Polak J . Colocalization of neuropeptide Y immunoreactivity in brainstem catecholaminergic neurons that project to the paraventricular nucleus of the hypothalamus. J Comp Neurol. 1985; 241(2):138-53. DOI: 10.1002/cne.902410203. View

13.

Kerkerian L, Salin P, Nieoullon A . Pharmacological characterization of dopaminergic influence on expression of neuropeptide Y immunoreactivity by rat striatal neurons. Neuroscience. 1988; 26(3):809-17. DOI: 10.1016/0306-4522(88)90101-7. View

14.

Giachetti A, Shore P . The reserpine receptor. Life Sci. 1978; 23(2):89-92. DOI: 10.1016/0024-3205(78)90254-0. View

15.

Heffner T, HARTMAN J, Seiden L . Feeding increases dopamine metabolism in the rat brain. Science. 1980; 208(4448):1168-70. DOI: 10.1126/science.7375926. View

16.

Stanley B, Magdalin W, Seirafi A, Thomas W, Leibowitz S . The perifornical area: the major focus of (a) patchily distributed hypothalamic neuropeptide Y-sensitive feeding system(s). Brain Res. 1993; 604(1-2):304-17. DOI: 10.1016/0006-8993(93)90382-w. View

17.

Li S, Pelletier G . The role of dopamine in the control of neuropeptide Y neurons in the rat arcuate nucleus. Neurosci Lett. 1986; 69(1):74-7. DOI: 10.1016/0304-3940(86)90417-9. View

18.

Stanley B, Leibowitz S . Neuropeptide Y injected in the paraventricular hypothalamus: a powerful stimulant of feeding behavior. Proc Natl Acad Sci U S A. 1985; 82(11):3940-3. PMC: 397905. DOI: 10.1073/pnas.82.11.3940. View

19.

Allen Y, Adrian T, Allen J, Tatemoto K, Crow T, Bloom S . Neuropeptide Y distribution in the rat brain. Science. 1983; 221(4613):877-9. DOI: 10.1126/science.6136091. View

20.

McDonald J, Lumpkin M, Samson W, McCANN S . Neuropeptide Y affects secretion of luteinizing hormone and growth hormone in ovariectomized rats. Proc Natl Acad Sci U S A. 1985; 82(2):561-4. PMC: 397080. DOI: 10.1073/pnas.82.2.561. View