Decrease in Cytochrome C Oxidase Activity Detected Cytochemically in the Placental Trophoblast of Patients with Pre-eclampsia

This study was designed to explore the possible relationship between cytochemically detectable cytochrome c oxidase (COX) activity in the trophoblast and placental dysfunction in patients with pre-eclampsia. Fifteen placentae (10 from women with an uncomplicated pregnancy and five from patients with pre-eclampsia) were involved in this study. Localization of COX activity in the placental trophoblast was determined by ultrastructural enzyme cytochemistry using DAB as a capturing agent. COX activity was observed in the intracristal spaces and intermembrane spaces of trophoblastic mitochondria. The number of mitochondria positive for COX staining was decreased markedly in the placentae of the women with pre-eclampsia. Trophoblastic mitochondrial dysfunction may be present in placenta of patients with pre-eclampsia.