RAP-1 is an Arabidopsis MYC-like R Protein Homologue, That Binds to G-box Sequence Motifs

Overview

Journal Plant Mol Biol

Date 1997 May 1

PMID 9177323

Citations 18

Authors

S de Pater

K Pham

J Memelink

J Kijne

Affiliations

Soon will be listed here.

Abstract

An Arabidopsis cDNA clone encoding a DNA-binding protein, RAP-1, was isolated by southwestern screening of an Escherichia coli cDNA expression library. The protein contains a bHLH DNA-binding domain and is homologous to R proteins, regulating anthocyanin biosynthesis. RAP-1 binds to the sequence CACNTG. It is encoded by a single gene, which is expressed to high levels in root and stem and to low levels in leaf and flower. No expression could be detected in siliques. Rap-1 does not correspond to one of the known loci involved in anthocyanin biosynthesis, since it is located at a different map position. In contrast to the maize R protein Lc, RAP-1 did not induce anthocyanin biosynthesis in pea cotyledons. Thus, RAP-1 is a novel member of the bHLH class of DNA-binding proteins.

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