Beta-Glucosylated Proteins in the Cell Wall of the Black Yeast Exophiala (Wangiella) Dermatitidis

Overview

Journal Microbiology (Reading)

Specialty Microbiology

Date 1997 May 1

PMID 9168616

Citations 9

Authors

Roy C Montijn

Patricia Van Wolven

Sybren de Hoog

Frans M Klis

Affiliations

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Abstract

Wild-type cells of the pathogenic black yeast Exophiala (Wangiella) dermatitidis grown in a low-pH ascorbate medium became less melanized and less resistant to Zymolyase. This was accompanied by increased staining with fluorescently labelled concanavalin A. The sugar composition of wild-type and mutant cell walls was, except for the presence of galactose, similar to that of Saccharomyces cerevisiae. Digestion of mutant cell walls with laminarinase released galactomannoproteins. In addition, the released cell wall proteins contained glucose and reacted with affinity-purified 1,6-beta-glucan antiserum, indicating that they are linked to 1,6-beta-glucan. It is proposed that 1,6-beta-glucosylated cell wall proteins generally occur among ascomycetes.

Citing Articles

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da Silva J, de Oliveira H, Marcos C, da Silva R, da Costa T, Calich V PLoS One. 2013; 8(4):e62533.

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Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae.

Yin Q, de Groot P, de Jong L, Klis F, de Koster C FEMS Yeast Res. 2007; 7(6):887-96.

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Zheng L, Mendoza L, Wang Z, Liu H, Park C, Kauffman S Arch Microbiol. 2006; 185(4):316-29.

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Liu H, Kauffman S, Becker J, Szaniszlo P Eukaryot Cell. 2004; 3(1):40-51.

PMID: 14871935 PMC: 329517. DOI: 10.1128/EC.3.1.40-51.2004.

Molecular cloning and characterization of WdPKS1, a gene involved in dihydroxynaphthalene melanin biosynthesis and virulence in Wangiella (Exophiala) dermatitidis.

Feng B, Wang X, Hauser M, Kaufmann S, Jentsch S, Haase G Infect Immun. 2001; 69(3):1781-94.

PMID: 11179356 PMC: 98085. DOI: 10.1128/IAI.69.3.1781-1794.2001.