Salicylic Acid Activates a 48-kD MAP Kinase in Tobacco

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1997 May 1

PMID 9165755

Citations 162

Authors

S Zhang

D F Klessig

Affiliations

Soon will be listed here.

Abstract

The involvement of phosphorylation/dephosphorylation in the salicylic acid (SA) signal transduction pathway leading to pathogenesis-related gene induction has previously been demonstrated using kinase and phosphatase inhibitors. Here, we show that in tobacco suspension cells, SA induced a rapid and transient activation of a 48-kD kinase that uses myelin basic protein as a substrate. This kinase is called the p48 SIP kinase (for SA-Induced Protein kinase). Biologically active analogs of SA, which induce pathogenesis-related genes and enhanced resistance, also activated this kinase, whereas inactive analogs did not. Phosphorylation of a tyrosine residue(s) in the SIP kinase was associated with its activation. The SIP kinase was purified to homogeneity from SA-treated tobacco suspension culture cells. The purified SIP kinase is strongly phosphorylated on a tyrosine residue(s), and treatment with either protein tyrosine or serine/threonine phosphatases abolished its activity. Using primers corresponding to the sequences of internal tryptic peptides, we cloned the SIP kinase gene. Analysis of the SIP kinase sequence indicates that it belongs to the MAP kinase family and that it is distinct from the other plant MAP kinases previously implicated in stress responses, suggesting that different members of the MAP kinase family are activated by different stresses.

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References

Herskowitz I . MAP kinase pathways in yeast: for mating and more. Cell. 1995; 80(2):187-97. DOI: 10.1016/0092-8674(95)90402-6. View

Dietrich A, Mayer J, Hahlbrock K . Fungal elicitor triggers rapid, transient, and specific protein phosphorylation in parsley cell suspension cultures. J Biol Chem. 1990; 265(11):6360-8. View

Despres C, Subramaniam R, Matton D, Brisson N . The Activation of the Potato PR-10a Gene Requires the Phosphorylation of the Nuclear Factor PBF-1. Plant Cell. 1995; 7(5):589-598. PMC: 160806. DOI: 10.1105/tpc.7.5.589. View

Popping B, GIBBONS T, Watson M . The Pisum sativum MAP kinase homologue (PsMAPK) rescues the Saccharomyces cerevisiae hog1 deletion mutant under conditions of high osmotic stress. Plant Mol Biol. 1996; 31(2):355-63. DOI: 10.1007/BF00021795. View

Wilson C, Eller N, Gartner A, Vicente O, Heberle-Bors E . Isolation and characterization of a tobacco cDNA clone encoding a putative MAP kinase. Plant Mol Biol. 1993; 23(3):543-51. DOI: 10.1007/BF00019302. View

Decroocq S, van WENT J, Schmidt E, Kreis M . A homologue of the MAP/ERK family of protein kinase genes is expressed in vegetative and in female reproductive organs of Petunia hybrida. Plant Mol Biol. 1995; 27(2):339-50. DOI: 10.1007/BF00020188. View

Kyriakis J, Avruch J . Protein kinase cascades activated by stress and inflammatory cytokines. Bioessays. 1996; 18(7):567-77. DOI: 10.1002/bies.950180708. View

Bogre L, Ligterink W, Heberle-Bors E, Hirt H . Mechanosensors in plants. Nature. 1996; 383(6600):489-90. DOI: 10.1038/383489a0. View

Jonak C, Pay A, Bogre L, Hirt H, Heberle-Bors E . The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner. Plant J. 1993; 3(4):611-7. DOI: 10.1046/j.1365-313x.1993.03040611.x. View

10.

Zhang S, Jin C, Roux S . Casein Kinase II-Type Protein Kinase from Pea Cytoplasm and Its Inactivation by Alkaline Phosphatase in Vitro. Plant Physiol. 1993; 103(3):955-962. PMC: 159069. DOI: 10.1104/pp.103.3.955. View

11.

Hunter T . Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. Cell. 1995; 80(2):225-36. DOI: 10.1016/0092-8674(95)90405-0. View

12.

Mizoguchi T, Hayashida N, Yamaguchi-Shinozaki K, Kamada H, Shinozaki K . ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana. FEBS Lett. 1993; 336(3):440-4. DOI: 10.1016/0014-5793(93)80852-l. View

13.

Boulton T, Yancopoulos G, Gregory J, Slaughter C, Moomaw C, Hsu J . An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control. Science. 1990; 249(4964):64-7. DOI: 10.1126/science.2164259. View

14.

Mizoguchi T, Gotoh Y, Nishida E, Yamaguchi-Shinozaki K, Hayashida N, Iwasaki T . Characterization of two cDNAs that encode MAP kinase homologues in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase activities in cultured cells. Plant J. 1994; 5(1):111-22. DOI: 10.1046/j.1365-313x.1994.5010111.x. View

15.

Usami S, Banno H, Ito Y, Nishihama R, Machida Y . Cutting activates a 46-kilodalton protein kinase in plants. Proc Natl Acad Sci U S A. 1995; 92(19):8660-4. PMC: 41026. DOI: 10.1073/pnas.92.19.8660. View

16.

Klessig D, Malamy J . The salicylic acid signal in plants. Plant Mol Biol. 1994; 26(5):1439-58. DOI: 10.1007/BF00016484. View

17.

Sun H, Tonks N . The coordinated action of protein tyrosine phosphatases and kinases in cell signaling. Trends Biochem Sci. 1994; 19(11):480-5. DOI: 10.1016/0968-0004(94)90134-1. View

18.

Felix G, Grosskopf D, Regenass M, Boller T . Rapid changes of protein phosphorylation are involved in transduction of the elicitor signal in plant cells. Proc Natl Acad Sci U S A. 1991; 88(19):8831-4. PMC: 52604. DOI: 10.1073/pnas.88.19.8831. View

19.

Childs T, Mak A . MAP kinases from bovine brain: purification and characterization. Biochem Cell Biol. 1993; 71(11-12):544-55. DOI: 10.1139/o93-078. View

20.

Duerr B, Gawienowski M, Ropp T, Jacobs T . MsERK1: a mitogen-activated protein kinase from a flowering plant. Plant Cell. 1993; 5(1):87-96. PMC: 160253. DOI: 10.1105/tpc.5.1.87. View