The Drosophila Gene Abnormal Spindle Encodes a Novel Microtubule-associated Protein That Associates with the Polar Regions of the Mitotic Spindle

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1997 May 19

PMID 9151690

Citations 64

Authors

R D Saunders

M C Avides

T Howard

C Gonzalez

D M Glover

Affiliations

Soon will be listed here.

Abstract

abnormal spindle, a gene required for normal spindle structure and function in Drosophila melanogaster, lies immediately adjacent the gene tolloid at 96A/B. It encodes a 220-kD polypeptide with a predicted pI of 10.8. The recessive mutant allele asp1 directs the synthesis of a COOH terminally truncated or internally deleted peptide of approximately 124 kD. Wild-type Asp protein copurifies with microtubules and is not released by salt concentrations known to dissociate most other microtubule-associated proteins. The bacterially expressed NH2-terminal 512-amino acid peptide, which has a number of potential phosphorylation sites for p34(cdc2) and MAP kinases, strongly binds to microtubules. The central 579-amino acid segment of the molecule contains one short motif homologous to sequences in a number of actin bundling proteins and a second motif present at the calmodulin binding sites of several proteins. Immunofluorescence studies show that the wild-type Asp protein is localized to the polar regions of the spindle immediately surrounding the centrosome. These findings are discussed in relation to the known spindle abnormalities in asp mutants.

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References

Fellous A, Prasad V, Ohayon R, Jordan M, Luduena R . Removal of the projection domain of microtubule-associated protein 2 alters its interaction with tubulin. J Protein Chem. 1994; 13(4):381-91. DOI: 10.1007/BF01901694. View

Stirling D, Welch K, Stark M . Interaction with calmodulin is required for the function of Spc110p, an essential component of the yeast spindle pole body. EMBO J. 1994; 13(18):4329-42. PMC: 395360. DOI: 10.1002/j.1460-2075.1994.tb06753.x. View

Itoh T, Hotani H . Microtubule-stabilizing activity of microtubule-associated proteins (MAPs) is due to increase in frequency of rescue in dynamic instability: shortening length decreases with binding of MAPs onto microtubules. Cell Struct Funct. 1994; 19(5):279-90. DOI: 10.1247/csf.19.279. View

Ookata K, Hisanaga S, Bulinski J, Murofushi H, Aizawa H, Itoh T . Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics. J Cell Biol. 1995; 128(5):849-62. PMC: 2120387. DOI: 10.1083/jcb.128.5.849. View

Gonzalez C, Molina I, Casal J, Ripoll P . Gross genetic dissection and interaction of the chromosomal region 95E;96F of Drosophila melanogaster. Genetics. 1989; 123(2):371-7. PMC: 1203808. DOI: 10.1093/genetics/123.2.371. View

Saunders R, Glover D, Ashburner M, Siden-Kiamos I, Louis C, Monastirioti M . PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning. Nucleic Acids Res. 1989; 17(22):9027-37. PMC: 335111. DOI: 10.1093/nar/17.22.9027. View

Bresnick A, Warren V, Condeelis J . Identification of a short sequence essential for actin binding by Dictyostelium ABP-120. J Biol Chem. 1990; 265(16):9236-40. View

Casal J, Gonzalez C, Wandosell F, Avila J, Ripoll P . Abnormal meiotic spindles cause a cascade of defects during spermatogenesis in asp males of Drosophila. Development. 1990; 108(2):251-60. DOI: 10.1242/dev.108.2.251. View

de Arruda M, Watson S, Lin C, Leavitt J, Matsudaira P . Fimbrin is a homologue of the cytoplasmic phosphoprotein plastin and has domains homologous with calmodulin and actin gelation proteins. J Cell Biol. 1990; 111(3):1069-79. PMC: 2116281. DOI: 10.1083/jcb.111.3.1069. View

10.

Bulinski J, Borisy G . Microtubule-associated proteins from cultured HeLa cells. Analysis of molecular properties and effects on microtubule polymerization. J Biol Chem. 1980; 255(23):11570-6. View

11.

Scalenghe F, Turco E, Edstrom J, Pirrotta V, Melli M . Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. Chromosoma. 1981; 82(2):205-16. DOI: 10.1007/BF00286105. View

12.

De Brabander M, Bulinski J, Geuens G, de Mey J, Borisy G . Immunoelectron microscopic localization of the 210,000-mol wt microtubule-associated protein in cultured cells of primates. J Cell Biol. 1981; 91(2 Pt 1):438-45. PMC: 2111990. DOI: 10.1083/jcb.91.2.438. View

13.

Saunders R . In situ hybridization to Drosophila polytene chromosomes. Methods Mol Biol. 1994; 33:183-91. DOI: 10.1385/0-89603-280-9:183. View

14.

Salisbury J . Centrin, centrosomes, and mitotic spindle poles. Curr Opin Cell Biol. 1995; 7(1):39-45. DOI: 10.1016/0955-0674(95)80043-3. View

15.

Lopez L, Sheetz M . A microtubule-associated protein (MAP2) kinase restores microtubule motility in embryonic brain. J Biol Chem. 1995; 270(21):12511-7. DOI: 10.1074/jbc.270.21.12511. View

16.

Finelli A, Xie T, Bossie C, Blackman R, PADGETT R . The tolkin gene is a tolloid/BMP-1 homologue that is essential for Drosophila development. Genetics. 1995; 141(1):271-81. PMC: 1206726. DOI: 10.1093/genetics/141.1.271. View

17.

Sundberg H, Goetsch L, Byers B, Davis T . Role of calmodulin and Spc110p interaction in the proper assembly of spindle pole body compenents. J Cell Biol. 1996; 133(1):111-24. PMC: 2120774. DOI: 10.1083/jcb.133.1.111. View

18.

Loveland K, Hayes T, Meinhardt A, Zlatic K, Parvinen M, de Kretser D . Microtubule-associated protein-2 in the rat testis: a novel site of expression. Biol Reprod. 1996; 54(4):896-904. DOI: 10.1095/biolreprod54.4.896. View

19.

Stirling D, Rayner T, Prescott A, Stark M . Mutations which block the binding of calmodulin to Spc110p cause multiple mitotic defects. J Cell Sci. 1996; 109 ( Pt 6):1297-310. DOI: 10.1242/jcs.109.6.1297. View

20.

Spang A, Courtney I, Grein K, Matzner M, Schiebel E . The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole body. J Cell Biol. 1995; 128(5):863-77. PMC: 2120402. DOI: 10.1083/jcb.128.5.863. View