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High-performance Thin-layer Chromatographic Determination of Psychopharmacologic Agents in Blood Serum

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Journal J Chromatogr
Date 1977 Nov 11
PMID 914927
Citations 4
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Abstract

High-performance thin-layer chromatography affords a rapid, sensitive method for determination of psychopharmacologic agents in blood serum samples. Quantitation of the representative drugs chlorpromazine, amitriptyline, nortriptyline, imipramine, and desipramine at levels as low as 5 ng/ml is demonstrated by scanning the developed thin-layer plates with a chromatographic spectrophotometer in the ultraviolet absorption mode. Neither derivatization prior to, nor color development after chromatographic separation is required to achieve sensitivity and reproducibility of determinations.

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