Colicin E3 Induced Cleavage of 16s RRNA of Isolated 30S Ribosomal Subunits

Overview

Journal J Biochem

Publisher Oxford University Press

Specialty Biochemistry

Date 1977 Sep 1

PMID 914815

Citations 3

Authors

Y Ohno-Iwashita

K Imahori

Affiliations

Soon will be listed here.

Abstract

Contrary to the previous findings we found that a specific cleavage of 16S rRNA occurred when protein A, the active component of colicin E3, was interacted with isolated 30S ribosomal subunits at high concentrations. Necessary experiments to disprove the possibility of artefacts are described.

Citing Articles

Structural basis for 16S ribosomal RNA cleavage by the cytotoxic domain of colicin E3.

Ng C, Lang K, Meenan N, Sharma A, Kelley A, Kleanthous C Nat Struct Mol Biol. 2010; 17(10):1241-1246.

PMID: 20852642 PMC: 3755339. DOI: 10.1038/nsmb.1896.

Colicin E3 cleavage of 16S rRNA impairs decoding and accelerates tRNA translocation on Escherichia coli ribosomes.

Lancaster L, Savelsbergh A, Kleanthous C, Wintermeyer W, Rodnina M Mol Microbiol. 2008; 69(2):390-401.

PMID: 18485067 PMC: 2615495. DOI: 10.1111/j.1365-2958.2008.06283.x.

Identification of the catalytic motif of the microbial ribosome inactivating cytotoxin colicin E3.

Walker D, Lancaster L, James R, Kleanthous C Protein Sci. 2004; 13(6):1603-11.

PMID: 15133158 PMC: 2279995. DOI: 10.1110/ps.04658504.