Bisulfite Reductase of Desulfovibrio Vulgaris: Explanation for Product Formation

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1977 Oct 1

PMID 914772

Citations 9

Authors

H L Drake

J M Akagi

Affiliations

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Abstract

Bisulfite reductase, purified from Desulfovibrio vulgaris, was coupled with the pyruvate phosphoroclastic reaction. Moderate to low reducing conditions resulted in the formation of trithionate; however, when the concentration of reductant was high, a mixture of trithionate and thiosulfate was formed. Sulfide was also a detectable product, but only when the concentration of bisulfite was low. Flavodoxin mediated native coupling between bisulfite reductase and the phosphoroclastic reaction. A model for bisulfite reductase activity is proposed.

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References

Akagi J, Campbell L . STUDIES ON THERMOPHILIC SULFATE-REDUCING BACTERIA III. : Adenosine Triphosphate-sulfurylase of Clostridium nigrificans and Desulfovibrio desulfuricans. J Bacteriol. 1962; 84(6):1194-201. PMC: 278045. DOI: 10.1128/jb.84.6.1194-1201.1962. View

Akagi J . PHOSPHOROCLASTIC REACTIONS OF CLOSTRIDIUM NIGRIFICANS. J Bacteriol. 1964; 88:813-4. PMC: 277389. DOI: 10.1128/jb.88.3.813-814.1964. View

Horio T, Kamen M . Preparation and properties of three pure crystalline bacterial haem proteins. Biochim Biophys Acta. 1961; 48:266-86. DOI: 10.1016/0006-3002(61)90476-0. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Drake H, Akagi J . Characterization of a novel thiosulfate-forming enzyme isolated from Desulfovibrio vulgaris. J Bacteriol. 1977; 132(1):132-8. PMC: 221836. DOI: 10.1128/jb.132.1.132-138.1977. View

Kobayashi K, Seki Y, Ishimoto M . Biochemical studies on sulfate-ruducing bacteria. 8. Sulfite reductase from Desulfovibrio vulgaris--mechanism of trithionate, thiosulfate, and sulfide formation and enzymatic properties. J Biochem. 1974; 75(3):519-29. DOI: 10.1093/oxfordjournals.jbchem.a130420. View

Jones H, SKYRING G . Effect of enzymic assay conditions on sulfite reduction catalysed by desulfoviridin from Desulfovibrio gigas. Biochim Biophys Acta. 1975; 377(1):52-60. DOI: 10.1016/0005-2744(75)90285-5. View

Drake H, Akagi J . Product analysis of bisulfite reductase activity isolated from Desulfovibrio vulgaris. J Bacteriol. 1976; 126(2):733-8. PMC: 233207. DOI: 10.1128/jb.126.2.733-738.1976. View

Akagi J, Chan M, Adams V . Observations on the bisulfite reductase (P582) isolated from Desulfotomaculum nigrificans. J Bacteriol. 1974; 120(1):240-4. PMC: 245756. DOI: 10.1128/jb.120.1.240-244.1974. View

10.

Kobayashi K, Takahashi E, Ishimoto M . Biochemical studies on sulfate-reducing bacteria. XI. Purification and some properties of sulfite reductase, desulfoviridin. J Biochem. 1972; 72(4):879-87. DOI: 10.1093/oxfordjournals.jbchem.a129982. View

11.

CHAMBERS L, TRUDINGER P . Are thiosulfate and trithionate intermediates in dissimilatory sulfate reduction?. J Bacteriol. 1975; 123(1):36-40. PMC: 235688. DOI: 10.1128/jb.123.1.36-40.1975. View

12.

Lee J, Peck Jr H . Purification of the enzyme reducing bisulfite to trithionate from Desulfovibrio gigas and its identification as desulfoviridin. Biochem Biophys Res Commun. 1971; 45(3):583-9. DOI: 10.1016/0006-291x(71)90457-8. View

13.

Suh B, Akagi J . Formation of thiosulfate from sulfite by Desulfovibrio vulgaris. J Bacteriol. 1969; 99(1):210-5. PMC: 249989. DOI: 10.1128/jb.99.1.210-215.1969. View

14.

Akagi J . Electron carries for the phosphoroclastic reaction of Desulfovibrio desulfuricans. J Biol Chem. 1967; 242(10):2478-83. View

15.

Suh B, Akagi J . Pyruvate-carbon dioxide exchange reaction of Desulfovibrio desulfuricans. J Bacteriol. 1966; 91(6):2281-5. PMC: 316207. DOI: 10.1128/jb.91.6.2281-2285.1966. View

16.

Brown M, Akagi J . Purification of acetokinase from Desulfovibrio desulfuricans. J Bacteriol. 1966; 92(4):1273-4. PMC: 276413. DOI: 10.1128/jb.92.4.1273-1274.1966. View