Gel Microdrop Technology for Rapid Isolation of Rare and High Producer Cells
Overview
Molecular Biology
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Secreted proteins are a therapeutic cornerstone of the biotechnology industry, and numerous recombinant products, including human growth hormone, human erythropoietin and granulocyte-colony stimulating factor, are now widely prescribed. In addition to bioprocessing applications, GMD technology should benefit the emerging fields of cellular and gene therapy. The ability to assess rapidly and precisely the productivity of cells for ex vivo cell screening and expansion is a unique approach with numerous research and therapeutic uses.
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Ghosh R, Arnheim A, van Zee M, Shang L, Soemardy C, Tang R Anal Chem. 2024; 96(20):7817-7839.
PMID: 38650433 PMC: 11112544. DOI: 10.1021/acs.analchem.4c01510.
Microfluidic chip-based single-cell cloning to accelerate biologic production timelines.
Diep J, Le H, Le K, Zasadzinska E, Tat J, Yam P Biotechnol Prog. 2021; 37(6):e3192.
PMID: 34323013 PMC: 9285370. DOI: 10.1002/btpr.3192.
Capture and display of antibodies secreted by hybridoma cells enables fluorescent on-cell screening.
Puligedda R, Sharma R, Al-Saleem F, Kouiavskaia D, Velu A, Kattala C MAbs. 2019; 11(3):546-558.
PMID: 30794061 PMC: 6512912. DOI: 10.1080/19420862.2019.1574520.
Functionalizing cell-mimetic giant vesicles with encapsulated bacterial biosensors.
Trantidou T, Dekker L, Polizzi K, Ces O, Elani Y Interface Focus. 2018; 8(5):20180024.
PMID: 30443325 PMC: 6227772. DOI: 10.1098/rsfs.2018.0024.
Fang Y, Chu T, Ackerman M, Griswold K MAbs. 2017; 9(8):1253-1261.
PMID: 28933630 PMC: 5680790. DOI: 10.1080/19420862.2017.1381812.