The Immunohistochemical Localization of Stat-2, -3, -4 and -5 During Early Enamel and Dentine Formation in Rat Molars

STATs (signal transduction and activators of transcription) are key components of the signal transduction pathways in the cytokine receptor superfamily-linked pathway. STATs are activated directly by members of the Jak (Janus kinase) family and, when activated, migrate to the nucleus to modify gene expression to produce a variety of cellular responses. Individual cytokines activate specific combinations of the Jak/STAT isoforms. A previous study localized the known Jak isoforms and STAT-1 in 5-day-old rat molars during the early stages of enamel and dentine formation. The present study was undertaken to localize immunohistochemically STAT isoforms STAT-2. -3, -4 and -5 in association with events involved in early dentine and enamel formation in 5-day-old rat molars. Each of the isoform localization patterns was different from the others. Combining the results of the previous study with the present findings, it appears that all of the known Jaks and STATs-1, -2, -3, -4 and -5 are located in the cells directly involved in early enamel or dentine formation. Using colocalization patterns of the individual Jaks and STATs, individual receptor locations may be predicted. In the proximal ends of differentiated ameloblasts, several cytokine receptors [interleukin (IL) -5, -6, -7, -9, -10, -12, growth hormone granulocyte colony-stimulating factor interferon-alpha/beta. -gamma] are predicted. In other areas of the early odontogenic cells, the proximal ends of differentiating ameloblasts are predicted to have IL-7 receptors, inner enamel epithelium IL-6 and IL-10 receptors, and stratum intermedium cells IL-6 receptors. In the early developing dentine, differentiating odontoblasts are predicted to have IL-6 and IL-10 receptors, and differentiated odontoblasts no cytokine receptors identified by known Jak/STAT combinations. Mapping of the Jak and STAT isoforms in the cells involved in early enamel and dentine formation indicates that a sizeable list of ligands and their respective cytokine receptor/pathway complexes are involved in the regulation of these processes.